State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, PR China.
State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, PR China.
Int J Biol Macromol. 2024 Nov;279(Pt 2):135293. doi: 10.1016/j.ijbiomac.2024.135293. Epub 2024 Sep 2.
Castor stalk from hemp plants is an attractive lignocellulosic feedstock for biomass refining valorization due to its similar chemical composition to hardwoods. In this study, the castor stalk fibers were pretreated with efficient dual-functional ethanolamine to achieve delignification and swelling of the cellulosic fibers, followed by cellulase enzymatic digestion for biomass conversion. Experimental results showed that ethanolamine pretreatment at 160 °C for 1 h effectively removed 69.20 % of lignin and 43.18 % of hemicellulose. In addition to efficient delignification and removal of hemicellulose, the study also revealed that supramolecular structure of cellulose was another major factor affecting enzymatic hydrolysis performance. The lowered crystallinity (60-70 %) and swelled crystal sizes (2.95-3.04 nm) promoted enzymatic hydrolysis efficiency during the heterogeneous reaction process. Under optimal conditions (160 °C, 1 h; enzyme loading: 15 FPU/g substrate), promoted yields of 100 % glucose and over 90 % xylose were achieved, which were significantly higher than those obtained from untreated castor stalk. These findings highlighted the effectiveness of the dual-functional ethanolamine pretreatment strategy for efficient bioconversion of lignocellulosic feedstocks. Overall, this study provides valuable insights into the development of new strategies for the efficient utilization of biomass resources, which is essential for the sustainable development of our society.
蓖麻秸秆来自大麻植物，由于其化学成分与硬木相似，是一种有吸引力的木质纤维素饲料原料，可用于生物质精炼增值。在这项研究中，采用高效双功能乙醇胺对蓖麻秸秆纤维进行预处理，实现了纤维素纤维的脱木质素和溶胀，然后用纤维素酶进行酶解转化生物质。实验结果表明，乙醇胺在 160°C 下预处理 1 小时可有效去除 69.20%的木质素和 43.18%的半纤维素。除了高效脱木质素和去除半纤维素外，该研究还表明，纤维素的超分子结构是影响酶解性能的另一个主要因素。结晶度降低（60-70%）和晶尺寸膨胀（2.95-3.04nm）促进了异相反应过程中的酶水解效率。在最佳条件下（160°C，1 小时；酶用量：15 FPU/g 底物），可实现 100%葡萄糖和 90%以上木糖的高转化率，明显高于未处理的蓖麻秸秆。这些发现强调了双功能乙醇胺预处理策略在木质纤维素饲料原料高效生物转化中的有效性。总的来说，本研究为开发高效利用生物质资源的新策略提供了有价值的见解，这对我们社会的可持续发展至关重要。
