Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.
Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.
Int J Biol Macromol. 2024 Nov;279(Pt 2):135291. doi: 10.1016/j.ijbiomac.2024.135291. Epub 2024 Sep 2.
Bacterial cellulose (BC) is an ideal candidate for wound dressings due to its natural origin, exceptional water-holding capacity, pliability, biocompatibility, and high absorption capability. However, the lack of essential antimicrobial activity limits its biomedical applications. This study reported BC-based wound dressings containing silk fibroin protein (SF), offering the potential for biomimetic properties, and (-)-epigallocatechin-3-gallate (EGCG) for polyphenol-assisted surface modification to promote infectious wound healing. Glycerol was used as the carbon source to promote the formation of an adhesive layer by facilitating the β-sheet folding of SF, and different concentrations of EGCG were employed to interact with SF through strong hydrogen bonding facilitated by the polyphenolic groups. The functionalized membrane exhibited outstanding water-holding capacity, swelling ratio, and degradation properties, along with enhanced hydrophilicity, adhesiveness, and a remarkable free radical scavenging ability. Both in vitro and in vivo experiments confirmed its potent bacteriostatic activity. The composite membrane displayed excellent biocompatibility, including cellular and hemocompatibility. Importantly, it effectively promoted wound healing in murine back infections. These findings suggest the significant feasibility of the innovative modification approach, and that functionalized membranes have great potential as wound-dressing materials for infection management in future clinical applications.
细菌纤维素（BC）由于其天然来源、出色的保水能力、柔韧性、生物相容性和高吸收能力，是理想的伤口敷料候选材料。然而，缺乏必要的抗菌活性限制了其在生物医学中的应用。本研究报道了一种基于 BC 的伤口敷料，其中含有丝素蛋白（SF），具有仿生特性的潜力，以及（-）-表没食子儿茶素-3-没食子酸酯（EGCG），用于多酚辅助的表面修饰，以促进感染性伤口愈合。甘油被用作碳源，通过促进 SF 的β-折叠，促进粘合层的形成，不同浓度的 EGCG 则通过多酚基团促进的氢键与 SF 相互作用。功能化膜表现出出色的持水能力、溶胀比和降解性能，以及增强的亲水性、粘附性和显著的自由基清除能力。体外和体内实验均证实了其具有强大的抑菌活性。复合膜表现出良好的生物相容性，包括细胞相容性和血液相容性。重要的是，它在小鼠背部感染模型中有效促进了伤口愈合。这些发现表明了这种创新改性方法的显著可行性，并且功能化膜作为未来临床应用中感染管理的伤口敷料材料具有巨大的潜力。
