School of Chemical Engineering and Technology, Xi'an Jiaotong University, Xi'an 710049, PR China; Xi'an Key Laboratory of C1 Compound Bioconversion Technology, Xi'an 710049, PR China.
School of Chemical Engineering and Technology, Xi'an Jiaotong University, Xi'an 710049, PR China.
Bioresour Technol. 2024 Nov;412:131415. doi: 10.1016/j.biortech.2024.131415. Epub 2024 Sep 2.
Bacterioruberin is widely used in medicine, food, and cosmetics owing to its prominent characteristics of antioxidants and bioactivities. Bioconversion of methane into bacterioruberin is a promising way to address biomanufacturing substrate costs and greenhouse gas emissions but has not been achieved yet. Herein, this study aimed to upcycle methane to bacterioruberin by microbial consortia. The microbial consortia consist of Methylomonas and Methylophilus capable of synthesizing carotenoids from methane was firstly enriched from paddy soil. Through this microbial community, methane was successfully converted into C50 bacterioruberin for the first time. The bioconversion process was then optimized by the response surface methodology. Finally, the methane-derived bacterioruberin reached a record yield of 280.88 ± 2.94 μg/g dry cell weight. This study presents a cost-effective and eco-friendly approach for producing long-chain carotenoids from methane, offering a significant advancement in the direct conversion of greenhouse gases into value-added products.
菌红菌素因其抗氧化和生物活性等突出特点，被广泛应用于医学、食品和化妆品领域。将甲烷生物转化为菌红菌素是解决生物制造底物成本和温室气体排放的一种很有前途的方法，但尚未实现。在此，本研究旨在通过微生物群落将甲烷循环利用为菌红菌素。首先，从稻田中富集了能够从甲烷合成类胡萝卜素的甲基单胞菌和甲基杆菌微生物群落。通过该微生物群落，甲烷首次成功转化为 C50 菌红菌素。然后通过响应面法对生物转化过程进行了优化。最后，甲烷衍生的菌红菌素的产量达到了 280.88±2.94μg/g 干细胞重量的记录水平。本研究为从甲烷生产长链类胡萝卜素提供了一种经济高效且环保的方法，为直接将温室气体转化为高附加值产品提供了重要进展。
