Noby Nehad, Khattab Sherine N, Soliman Nadia A
Department of Biotechnology, Institute of Graduate Studies and Research, Alexandria University, Alexandria, 21526, Egypt.
Department of Chemistry, Faculty of Science, Alexandria University, Alexandria, 21321, Egypt.
Bioresour Bioprocess. 2023 Jul 24;10(1):46. doi: 10.1186/s40643-023-00662-3.
Bacterioruberin and its rare glycosylated derivatives are produced by Arthrobacter agilis as an adaptation strategy to low temperature conditions. The high antioxidant properties of bacterioruberin held great promise for different future applications like the pharmaceutical and food industries. Microbial production of bacterioruberin via a cost-effective medium will help increase its commercial availability and industrial use. The presented study aims to optimize the production of the rare C carotenoid bacterioruberin and its derivatives from the psychotrophic bacteria Arthrobacter agilis NP20 strain on a whey-based medium as a cost effective and readily available nutritious substrate. The aim of the study is extended to assess the efficiency of whey treatment in terms of estimating total nitrogen content in treated and untreated whey samples. The significance of medium ingredients on process outcome was first tested individually; then the most promising factors were further optimized using Box Behnken design (BBD). The produced carotenoids were characterized using UV-visible spectroscopy, FTIR spectroscopy, HPLC-DAD chromatography and HPLC-APCI-MS spectrometry. The maximum pigment yield (5.13 mg/L) was achieved after a 72-h incubation period on a core medium composed of 96% sweet whey supplemented with 0.46% MgSO & 0.5% yeast extract and inoculated with 6% (v/v) of a 24 h pre-culture (10 CFU/mL). The cost of the formulated medium was 1.58 $/L compared with 30.1 $/L of Bacto marine broth medium. The extracted carotenoids were identified as bacterioruberin, bis-anhydrobacteriouberin, mono anhydrobacterioruberin, and glycosylated bacterioruberin. The presented work illustrates the possibility of producing bacterioruberin carotenoid from Arthrobacter agilis through a cost-effective and eco-friendly approach using cheese whey-based medium.
嗜冷节杆菌将细菌红素及其罕见的糖基化衍生物作为对低温条件的一种适应策略来产生。细菌红素的高抗氧化特性在制药和食品工业等不同的未来应用中具有很大的前景。通过具有成本效益的培养基微生物生产细菌红素将有助于提高其商业可得性和工业用途。本研究旨在优化从嗜冷节杆菌NP20菌株在基于乳清的培养基上生产罕见的C类胡萝卜素细菌红素及其衍生物，该培养基是一种具有成本效益且易于获得的营养底物。该研究的目的还扩展到在估计处理过和未处理过的乳清样品中的总氮含量方面评估乳清处理的效率。首先单独测试培养基成分对工艺结果的重要性；然后使用Box Behnken设计（BBD）进一步优化最有前景的因素。使用紫外可见光谱、傅里叶变换红外光谱、高效液相色谱-二极管阵列检测色谱和高效液相色谱-大气压化学电离质谱对产生的类胡萝卜素进行表征。在由96%甜乳清补充0.46%硫酸镁和0.5%酵母提取物组成的核心培养基上孵育72小时后，接种6%（v/v）的24小时预培养物（10CFU/mL），实现了最大色素产量（5.13mg/L）。配制培养基的成本为1.58美元/升，而Bacto海洋肉汤培养基的成本为30.1美元/升。提取的类胡萝卜素被鉴定为细菌红素、双脱水细菌红素、单脱水细菌红素和糖基化细菌红素。本研究表明了通过使用基于奶酪乳清的培养基以具有成本效益和生态友好的方法从嗜冷节杆菌生产细菌红素类胡萝卜素的可能性。
