Department of Clinical Sciences and Community Health, University of Milan, Milan, Italy.
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, NICU, Milan, Italy.
J Pediatr Gastroenterol Nutr. 2024 Nov;79(5):1024-1030. doi: 10.1002/jpn3.12364. Epub 2024 Sep 4.
The fatty acid (FA) supply of human milk (HM) contributes to health outcomes. Sampling fresh HM to analyze its FA content is challenging because of its ever-changing nature. Also, obtaining samples from lactating mothers is challenging. Facilitating HM collection and analysis is therefore an advantage.
We have conducted a study to validate a new method for obtaining HM samples for FA analysis, using biological fluid sample collection pretreated sheets to adsorb drops of milk (Whatman 903 BHT-pretreated biological fluid collection sheet) as an alternative approach to collecting expressed milk. The study population included lactating mothers, enrolled between 24 and 96 h after delivery.
A total of 124 breastmilk samples were analyzed using the two distinct approaches. The results of the free milk analysis were comparable to the analysis of adsorbed milk samples. The FA families saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), polyunsaturated fatty acids (PUFA), omega-3, and omega-6 had r values of 0.93, 0.91, 0.91, 0.86, and 0.90, respectively. Bland-Altman plots showed a high agreement between fresh and adsorbed milk samples for SFA, MUFA, PUFA, omega-3, and omega-6 with a mean bias <2% and 95% limits of agreement within -5% and +5%.
The results show no significant differences in fatty acid composition between fresh and adsorbed milk samples, suggesting the new method is equally effective in collecting representative samples for analysis.
人乳（HM）中的脂肪酸（FA）供应有助于健康结果。由于其不断变化的性质，采样新鲜 HM 来分析其 FA 含量具有挑战性。此外，从哺乳期母亲获得样本也具有挑战性。因此，方便 HM 采集和分析是一个优势。
我们进行了一项研究，以验证一种新的方法，用于获得用于 FA 分析的 HM 样本，使用预处理的生物流体样本采集片来吸附奶滴（Whatman 903 BHT 预处理生物流体采集片），作为收集表达奶的替代方法。研究人群包括分娩后 24 至 96 小时内的哺乳期母亲。
使用两种不同的方法分析了 124 个母乳样本。游离奶分析的结果与吸附奶样本的分析结果相当。饱和脂肪酸（SFA）、单不饱和脂肪酸（MUFA）、多不饱和脂肪酸（PUFA）、ω-3 和 ω-6 脂肪酸家族的 r 值分别为 0.93、0.91、0.91、0.86 和 0.90。Bland-Altman 图显示，新鲜奶和吸附奶样本在 SFA、MUFA、PUFA、ω-3 和 ω-6 方面具有高度一致性，平均偏差<2%，95%一致性界限在-5%和+5%范围内。
结果表明，新鲜奶和吸附奶样本的脂肪酸组成没有显著差异，表明新方法在收集代表性样本进行分析方面同样有效。
