Yang Seoin, Youn Wongu, Rheem Hyeong Bin, Han Sang Yeong, Kim Nayoung, Han Sol, Schattling Philipp, Städler Brigitte, Choi Insung S
Department of Chemistry, KAIST, 34141, Daejeon, South Korea.
Interdisciplinary Nanoscience Center, iNANO), Aarhus University, Gustav Wieds Vej 14, 8000, Aarhus, Denmark.
Angew Chem Int Ed Engl. 2025 Jan 15;64(3):e202415823. doi: 10.1002/anie.202415823. Epub 2024 Oct 29.
Integration of living cells with extrinsic functional entities gives rise to bioaugmented nanobiohybrids, which hold tremendous potential across diverse fields such as cell therapy, biocatalysis, and cell robotics. This study presents a biocompatible method for incorporating multilayered functional liposomes onto the cell surface, creating extracellular artificial organelles or exorganelles. The introduction of various extrinsic functionalities to cells is achieved without comprising their viabilities. The integration of extrinsic enzymatic reactions is exemplified through the cascade reaction involving glucose oxidase and horseradish peroxidase. Furthermore, our protocol offers the design flexibility to customize liposome compositions, thereby providing effective cell modification. The versatility of the liposome-based exorganelle approach establishes an advanced chemical tool, empowering cells with novel functionalities that surpass or are complementary to their innate capabilities.
活细胞与外在功能实体的整合产生了生物增强纳米生物杂交体，其在细胞治疗、生物催化和细胞机器人等不同领域具有巨大潜力。本研究提出了一种将多层功能脂质体整合到细胞表面的生物相容性方法，从而创建细胞外人工细胞器或外细胞器。在不影响细胞活力的情况下实现了将各种外在功能引入细胞。通过涉及葡萄糖氧化酶和辣根过氧化物酶的级联反应举例说明了外在酶促反应的整合。此外，我们的方案提供了设计灵活性以定制脂质体组成，从而实现有效的细胞修饰。基于脂质体的外细胞器方法的多功能性建立了一种先进的化学工具，赋予细胞超越其固有能力或与其互补的新功能。
