用核糖核酸酶消化法研究大鼠肝脏5S核糖体RNA-蛋白质L5复合物中的蛋白质-RNA相互作用。
Protein--RNA interaction in the rat liver 5S rRNA-protein L5 complex studied by digestion with ribonucleases.
作者信息
Gross B, Welfle H, Bielka H
出版信息
Nucleic Acids Res. 1985 Apr 11;13(7):2325-35. doi: 10.1093/nar/13.7.2325.
Abstract
Protein-RNA interactions in the 5S rRNA-protein L5 complex from rat liver ribosomes were studied by limited digestion of free and protein bound 5S rRNA with ribonuclease A and T1. In the complex with protein L5 the digestion of 5S rRNA by ribonuclease T1 is decreased at G37 and G89, whereas U38 and C39, and to a lower extent also C10 and U12 become accessible for ribonuclease A.
摘要
通过用核糖核酸酶A和T1对游离的和与蛋白质结合的5S核糖体RNA进行有限消化，研究了大鼠肝脏核糖体5S核糖体RNA-蛋白质L5复合物中的蛋白质-RNA相互作用。在与蛋白质L5形成的复合物中，核糖核酸酶T1对5S核糖体RNA在G37和G89处的消化减少，而U38和C39以及程度较低的C10和U12可被核糖核酸酶A作用。
Zotero 插件