Department of Neurology, Gongyi People's Hospital, No. 117 Renmin Road, Gongyi 451200, China.
Department of Neurological Rehabilitation, Gongyi People's Hospital, No. 117 Renmin Road, Gongyi 451200, China.
Biomed Res Int. 2021 Aug 6;2021:5512518. doi: 10.1155/2021/5512518. eCollection 2021.
Hyperlipidemia causes nervous system-related diseases. Exercise training has developed into an established evidence-based treatment strategy that is beneficial for neuronal injury. This study investigated the effect of exercise on hyperlipidemia-induced neuronal injury in apolipoprotein E-deficient (ApoE) mice. Male ApoE mice (age: 8 weeks) were randomly divided into four groups as follows: mice fed a normal diet (ND), normal diet+swimming training (ND+S), high-fat diet (HD), and high-fat diet+swimming (HD+S). Exercise training consisted of swimming for 40 min/day, 5 days/week for 12 weeks. After 12 weeks, we measured serum levels of total cholesterol (TC), triglyceride (TG), and low-density lipoprotein cholesterol (LDL-c). We also evaluated glial fibrillary acidic protein (GFAP) expression levels using immunohistochemistry, real-time PCR, and immunoblotting. In addition, NLR family pyrin domain-containing 3 (NLRP3), interleukin- (IL-) 18, caspase-1, Bax, Bcl-2, and phosphorylated extracellular signal-regulated kinase (p-ERK) expression levels were measured using immunoblotting. Serum levels of TG, TC, and LDL-c were lower in ApoE HD+S mice than in ApoE HD mice. Immunohistochemistry, real-time PCR, and immunoblotting showed increased levels of GFAP in the ApoE HD group. Immunoblotting revealed increased levels of NLRP3, IL-18, caspase-1, Bax, Bcl-2, and p-ERK in the ApoE HD group; however, they were significantly suppressed in the ApoE HD+S group. Therefore, exercise has protective effects against neuronal injury caused by hyperlipidemia.
高脂血症引起神经系统相关疾病。运动训练已发展成为一种既定的循证治疗策略，有利于神经元损伤。本研究探讨了运动对载脂蛋白 E 缺陷（ApoE）小鼠高脂血症诱导的神经元损伤的影响。雄性 ApoE 小鼠（年龄：8 周）随机分为四组：正常饮食组（ND）、正常饮食+游泳训练组（ND+S）、高脂饮食组（HD）和高脂饮食+游泳训练组（HD+S）。运动训练包括每天游泳 40 分钟，每周 5 天，共 12 周。12 周后，我们测量了血清总胆固醇（TC）、甘油三酯（TG）和低密度脂蛋白胆固醇（LDL-c）水平。我们还通过免疫组织化学、实时 PCR 和免疫印迹法评估了胶质纤维酸性蛋白（GFAP）的表达水平。此外，还通过免疫印迹法测量了 NOD 样受体家族含pyrin 结构域蛋白 3（NLRP3）、白细胞介素-18（IL-18）、半胱天冬酶-1（caspase-1）、Bax、Bcl-2 和磷酸化细胞外信号调节激酶（p-ERK）的表达水平。与 ApoE HD 组相比，ApoE HD+S 组小鼠血清 TG、TC 和 LDL-c 水平降低。免疫组织化学、实时 PCR 和免疫印迹法显示 ApoE HD 组 GFAP 水平升高。免疫印迹法显示 ApoE HD 组 NLRP3、IL-18、caspase-1、Bax、Bcl-2 和 p-ERK 水平升高，但在 ApoE HD+S 组明显抑制。因此，运动对高脂血症引起的神经元损伤具有保护作用。
