Faculty of Science, Kunming University of Science and Technology, Kunming, Yunnan, China.
Medical School, Kunming University of Science and Technology, Kunming, Yunnan, China.
Appl Environ Microbiol. 2024 Oct 23;90(10):e0228723. doi: 10.1128/aem.02287-23. Epub 2024 Sep 5.
The composition and stability of the microbial community structure of roots and root zone soils play a key role in the healthy growth of plants. We examined the distribution characteristics of phenolic acids and saponins, as well as microbial communities in the root space (root endosphere, rhizoplane soil, rhizosphere soil, and bulk soil) of healthy and root rot disease-affected . The results showed that after infection with root rot, the rhizoplane soil exhibited significant decreases in organic matter and hydrolyzable nitrogen and significant increases in available phosphorus, available potassium, and total nitrogen. The contents of phenolic acids (except benzoic acid) and ginsenoside Rg2 in the root endosphere significantly increased. Ferulic acid and p-hydroxybenzoic acid in the rhizoplane soil significantly increased. increased significantly in the root endosphere, rhizoplane, and rhizosphere soil; decreased significantly in the rhizoplane, rhizosphere, and bulk soil; and decreased significantly in the root endosphere and rhizoplane soil. Moreover, the accumulation of most autotoxins can promote the growth of pathogens. In summary, the spatial autotoxic substances and microbial community differences in roots jointly induce the occurrence of root rot.IMPORTANCE is highly susceptible to soil-borne diseases induced during planting, and root rot, which usually occurs in the root and stem parts of the plant, is the most severe. We divided the root environment of into four parts (root endosphere, rhizoplane soil, rhizosphere soil, and bulk soil) and studied it with unplanted soil as the control. In this study, we examined the changes in the content of autotoxic substances in the root space of , along with the interplay between these substances and microorganisms. This study revealed the mechanism underlying root rot and provided a theoretical basis for alleviating continuous cropping obstacles in .
根系及根区土壤微生物群落结构的组成和稳定性对植物的健康生长起着关键作用。我们研究了健康和根腐病感染的根系空间（根内圈、根际土壤、根围土壤和原状土壤）中酚酸和皂苷的分布特征以及微生物群落。结果表明，根腐病感染后，根际土壤有机质和水解氮显著减少，有效磷、有效钾和全氮显著增加。根内圈中酚酸（苯甲酸除外）和人参皂苷 Rg2 的含量显著增加。根际土壤中阿魏酸和对羟基苯甲酸含量显著增加。根内圈、根际和根围土壤中 含量显著增加；根际、根围和原状土壤中 含量显著降低；根内圈和根际土壤中 含量显著降低。此外，大多数自毒物质的积累可以促进病原菌的生长。综上所述， 根中空间自毒物质和微生物群落的差异共同诱导根腐病的发生。重要的是，在种植过程中易受土传病害的影响，而根腐病通常发生在植物的根和茎部，是最严重的病害。我们将 的根环境分为四个部分（根内圈、根际土壤、根围土壤和原状土壤），并以未种植的土壤作为对照进行研究。在本研究中，我们研究了根空间中自毒物质含量的变化，以及这些物质与微生物之间的相互作用。本研究揭示了根腐病的发生机制，为缓解 连作障碍提供了理论依据。
