Immunotechnology Lab, Department of Biotechnology, University of Calicut, Malappuram, 673635, Kerala, India.
Department of Integrated Biology, St. Joseph's College (Autonomous), Irinjalakuda, Thrissur, 680121, Kerala, India.
Biometals. 2024 Oct;37(5):1289-1303. doi: 10.1007/s10534-024-00610-4. Epub 2024 Sep 5.
Green synthesis of iron oxide nanoparticles using plant extracts is of tremendous interest owing to its cost effectiveness, ecofriendly and high efficiency compared to physical and chemical approaches. In the current study, we describe a green approach for producing iron oxide nanoparticles utilizing Polyalthia korintii aqueous leaf extract (PINPs). The prepared PINPs were assessed of their biological and dye degradation potentials. The physico-chemical characterization of PINPs using UV-Visible spectrophotometer, Fourier Transform Infrared Spectroscopy, X-Ray Diffraction studies, Field emission Scanning Electron Microscopy and Energy Dispersive X-ray spectroscopy analysis confirmed the synthesized sample comprised of iron oxide entity, predominantly spherical with the size range of 40-60 nm. Total Phenolic Content of PINPs is 59.36 ± 1.64 µg GAE/mg. The PINPs exhibited 89.78 ± 0.07% DPPH free radical scavenging and 28.7 ± 0.21% ABTS cation scavenging activities. The antibacterial activities were tested against different gram-positive and gram-negative bacteria and PINPs were more effective against Enterococcus faecalis and Klebsiella pneumoniae. Cytotoxicity of PINPs against K562 and HCT116 were measured and IC50 values were found to be 84.99 ± 4.3 µg/ml and 79.70 ± 6.2 µg/ml for 48 h respectively. The selective toxicity of PINPs was demonstrated by their lowest activity on lymphocytes, HEK293 cells, and erythrocytes. The toxicity (LC 50 values) against first, second, third and fourth instar larvae of Culex quinquefasciatus was 40 ± 1.5 mg/mL, 45 ± 0.8 mg/mL, 99 ± 2.1 mg/mL and 120 ± 3.5 mg/mL respectively. Finally, PINPs were utilized to as a catalyst for removal of textile dyes like Methylene blue and methyl orange in a fenton-like reaction. The results showed 100% dye degradation efficiency in a fenton like reaction within 35 min. Thus, the green synthesized PINPs exhibit antioxidant, antibacterial, antiproliferative, larvicidal and dye degradation potentials, indicating their suitability for biological and environmental applications.
利用植物提取物合成氧化铁纳米粒子因其成本效益高、环保且比物理和化学方法效率更高而备受关注。在本研究中，我们描述了一种利用 Polyalthia korintii 水提叶提取物（PINPs）制备氧化铁纳米粒子的绿色方法。评估了制备的 PINPs 的生物和染料降解潜力。通过紫外-可见分光光度计、傅里叶变换红外光谱、X 射线衍射研究、场发射扫描电子显微镜和能量色散 X 射线能谱分析对 PINPs 的物理化学特性进行了表征，证实合成的样品由氧化铁实体组成，主要为 40-60nm 的球形。PINPs 的总酚含量为 59.36±1.64μg GAE/mg。PINPs 表现出 89.78±0.07%DPPH 自由基清除活性和 28.7±0.21%ABTS 阳离子清除活性。抗菌活性测试针对不同的革兰氏阳性菌和革兰氏阴性菌，结果表明 PINPs 对粪肠球菌和肺炎克雷伯菌更有效。测定了 PINPs 对 K562 和 HCT116 的细胞毒性，发现其 48 小时的 IC50 值分别为 84.99±4.3μg/ml 和 79.70±6.2μg/ml。通过其对淋巴细胞、HEK293 细胞和红细胞的最低活性证明了 PINPs 的选择性毒性。对第一、二、三、四代库蚊幼虫的毒性（LC50 值）分别为 40±1.5mg/mL、45±0.8mg/mL、99±2.1mg/mL 和 120±3.5mg/mL。最后，PINPs 被用作芬顿反应中去除亚甲蓝和甲基橙等纺织染料的催化剂。结果表明，在芬顿反应中，35 分钟内染料的降解效率达到 100%。因此，绿色合成的 PINPs 表现出抗氧化、抗菌、抗增殖、杀幼虫和染料降解潜力，表明其适用于生物和环境应用。
