Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, PL-60965 Poznan, Poland.
Institute of Chemistry and Technical Electrochemistry, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, PL-60965 Poznan, Poland.
Int J Biol Macromol. 2019 Apr 15;127:677-682. doi: 10.1016/j.ijbiomac.2019.02.008. Epub 2019 Feb 3.
Glucose monitoring has become a crucial part of diabetes care, and is also of importance in the food industry. However, the measurements are subject to certain limitations and errors. These may result from many factors, including the strip manufacturing process, inappropriate storage, temperature, coding, aging, etc. Here, we discuss the measurement of glucose in real samples using techniques such as photometric assay, glucometers, and using a proposed biosensor. Biosensor platforms based on the multicomponent material magnetite/lignin/polydopamine-glucose oxidase with the addition of ferrocene and a dedicated carbon paste electrode (CPE/FeO/Lig/PDA/GOx/Fc) were fabricated for glucose detection in real, commercially available glucose-based samples. To determine the morphological features of the materials, Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), atomic force microscopy (AFM) and zeta potential measurements were carried out. Moreover, to determine the effectiveness of glucose oxidase immobilization the Bradford assays was applied. Analysis of glucose-based products was carried out based on photometric measurements using an AU480 Chemical Analyzer from Beckam Coulter, with the use of three glucometers (Wellion Calla light® WELL 900LB, CERA-CHEK 1Code®Model G400, iXell®Rev.04/10-PL), and with the use of our proposed CPE/FeO/Lig/PDA/GOx/Fc biosensor. Tests on real samples demonstrated the repeatability of the results. The results showed that this biosensor has excellent potential for application in the determination of glucose in various commercial products.
血糖监测已成为糖尿病治疗的重要组成部分，在食品工业中也很重要。然而，测量结果存在一定的局限性和误差。这些可能是由许多因素引起的，包括条带制造过程、储存不当、温度、编码、老化等。在这里，我们讨论了使用比色法、血糖仪以及使用提出的生物传感器来测量实际样品中的葡萄糖。我们制备了基于多组分材料磁铁矿/木质素/聚多巴胺-葡萄糖氧化酶的生物传感器平台，其中添加了二茂铁，并使用专用的碳糊电极（CPE/FeO/Lig/PDA/GOx/Fc），用于检测实际的、市售的基于葡萄糖的样品中的葡萄糖。为了确定材料的形态特征，进行了傅里叶变换红外光谱（FTIR）、透射电子显微镜（TEM）、原子力显微镜（AFM）和zeta 电位测量。此外，为了确定葡萄糖氧化酶固定化的效果，应用了 Bradford 测定法。基于 Beckam Coulter 的 AU480 化学分析仪进行了基于葡萄糖的产品的光度分析，使用了三种血糖仪（Wellion Calla light® WELL 900LB、CERA-CHEK 1Code®Model G400、iXell®Rev.04/10-PL），并使用了我们提出的 CPE/FeO/Lig/PDA/GOx/Fc 生物传感器。对实际样品的测试表明，结果具有良好的重复性。结果表明，该生物传感器在各种商业产品中测定葡萄糖具有优异的应用潜力。
