Department of Physiology, Maj Institute of Pharmacology, Polish Academy of Sciences, Krakow, Poland.
Cancer Neurophysiology Group, Łukasiewicz - PORT, Polish Center for Technology Development, Stabłowicka 147, Wrocław, 54-066, Poland.
Pharmacol Rep. 2024 Dec;76(6):1242-1255. doi: 10.1007/s43440-024-00646-w. Epub 2024 Sep 5.
In recent years, fluorescent sensors are enjoying a surge of popularity in the field of neuroscience. Through the development of novel genetically encoded sensors as well as improved methods of detection and analysis, fluorescent sensing has risen as a new major technique in neuroscience alongside molecular, electrophysiological, and imaging methods, opening up new avenues for research. Combined with multiphoton microscopy and fiber photometry, these sensors offer unique advantages in terms of cellular specificity, access to multiple targets - from calcium dynamics to neurotransmitter release to intracellular processes - as well as high capability for in vivo interrogation of neurobiological mechanisms underpinning behavior. Here, we provide a brief overview of the method, present examples of its integration with other tools in recent studies ranging from cellular to systems neuroscience, and discuss some of its principles and limitations, with the aim of introducing new potential users to this rapidly developing and potent technique.
近年来，荧光传感器在神经科学领域中越来越受欢迎。通过新型基因编码传感器的开发以及检测和分析方法的改进，荧光传感已经成为神经科学中的一项新技术，与分子、电生理和成像方法并驾齐驱，为研究开辟了新的途径。结合多光子显微镜和光纤光度法，这些传感器在细胞特异性、能够同时检测多个目标（从钙动力学到神经递质释放再到细胞内过程）以及对行为背后的神经生物学机制进行体内研究方面具有独特的优势。在这里，我们简要概述了该方法，介绍了近年来从细胞到系统神经科学研究中与其他工具相结合的实例，并讨论了该方法的一些原理和局限性，目的是向新的潜在用户介绍这项快速发展且功能强大的技术。
