Laboratorio de Microbiología de Sistemas, Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago, Chile.
Grupo de Microbiología Integrativa, Laboratorio de Biología Estructural y Molecular BEM, Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago, Chile.
Methods Mol Biol. 2025;2852:181-196. doi: 10.1007/978-1-0716-4100-2_13.
This chapter introduces protocols for culturing and maintaining Dictyostelium discoideum and methods for conducting virulence assays in this organism to study bacterial pathogenicity. It outlines advanced techniques, such as automated microscopy and flow cytometry, for detailed cellular analysis and traditional microbiological approaches. These comprehensive protocols will enable researchers to probe the virulence factors of pathogens like Klebsiella pneumoniae and to elucidate the details of host-pathogen interactions within a cost-effective and adaptable laboratory framework.
本章介绍了培养和维持盘基网柄菌（Dictyostelium discoideum）的方案，以及在该生物体中进行毒力测定以研究细菌致病性的方法。它概述了先进的技术，如自动化显微镜和流式细胞术，用于详细的细胞分析和传统的微生物方法。这些全面的方案将使研究人员能够研究肺炎克雷伯菌（Klebsiella pneumoniae）等病原体的毒力因子，并在具有成本效益和适应性的实验室框架内阐明宿主-病原体相互作用的细节。
