Team of Biotechnology & Sustainable Development of Natural Resources, Department of Biology, Polydisciplinary Faculty, Sultan Moulay Slimane University, Beni Mellal, Morocco; Molecular Bacteriology Laboratory, Pasteur Institute of Morocco, Casablanca, Morocco.
Team of Biotechnology & Sustainable Development of Natural Resources, Department of Biology, Polydisciplinary Faculty, Sultan Moulay Slimane University, Beni Mellal, Morocco.
Diagn Microbiol Infect Dis. 2024 Dec;110(4):116483. doi: 10.1016/j.diagmicrobio.2024.116483. Epub 2024 Aug 8.
This study aimed to establish the correlation between antibiotic resistance and biofilm formation by Staphylococcus haemolyticus and to examine the impact of sub-inhibitory concentrations of antibiotics (sub-MICs) on biofilm formation. Antibiotic susceptibility testing was conducted using the disk diffusion method, and biofilm formation was determined using Congo red agar and microtiter plate methods. Antibiotic resistance and biofilm-associated genes were detected using polymerase chain reaction. The majority of the twenty-one S. haemolyticus isolates were multidrug-resistant, methicillin-resistant (MRSH) and biofilm producers, including 43 % of moderate biofilm producers. A significant correlation was observed between MRSH and MSSH isolates in terms of biofilm production. Vancomycin, gentamicin, and ciprofloxacin at their sub-MICs tended to promote biofilm formation. The eno gene was present in 76.2 % of strains, followed by aap, and atlE. This study revealed a strong correlation between the biofilm-forming ability and antibiotic resistance in S. haemolyticus, which underlines a crucial public health issue.
本研究旨在建立溶血性葡萄球菌的抗生素耐药性与生物膜形成之间的相关性，并研究亚抑菌浓度抗生素（sub-MICs）对生物膜形成的影响。采用纸片扩散法进行抗生素药敏试验，用刚果红琼脂和微量板法检测生物膜形成。采用聚合酶链反应检测抗生素耐药性和生物膜相关基因。在 21 株溶血性葡萄球菌分离株中，大多数为多药耐药、耐甲氧西林（MRSH）和生物膜生产者，其中包括 43%的中度生物膜生产者。MRSH 和 MSSH 分离株在生物膜形成方面存在显著相关性。万古霉素、庆大霉素和环丙沙星在亚抑菌浓度下可促进生物膜形成。eno 基因存在于 76.2%的菌株中，其次是 aap 和 atlE。本研究揭示了溶血性葡萄球菌生物膜形成能力与抗生素耐药性之间的强相关性，这突显了一个重要的公共卫生问题。
