Bio Team, Animal Industry Data Korea, Seoul 06152, Korea.
Laboratory of Veterinary Internal Medicine, BK21 FOUR Future Veterinary Medicine Leading Education and Research Centre, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
J Vet Sci. 2024 Sep;25(5):e59. doi: 10.4142/jvs.24148. Epub 2024 Aug 6.
Despite advancements in herd management, feeding, and pharmaceutical interventions, neonatal calf diarrhea (NCD) remains a major global health concern. Bacteria, viruses, and parasites are the major contributors to NCD. Although several pathogens have been identified in the Republic of Korea (ROK), the etiological agents of numerous NCD cases have not been identified.
To identify, for the first time, the prevalence and impact of (BooV) on calf diarrhea in the ROK.
Here, the unknown cause of calf diarrhea was determined using metagenomics We then explored the prevalence of certain pathogens, including BooV, that cause NCD. Seventy diarrheal fecal samples from Hanwoo () calves were analyzed using reverse transcriptase and quantitative real-time polymerase chain reaction for pathogen detection and BooV isolate sequencing.
The complete genome of BooV was detected from unknown causes of calf diarrhea. And also, BooV was the most frequently detected pathogen (35.7%) among 8 pathogens in 70 diarrheic feces from Hanwoo calves. Co-infection analyses indicated that most BooV-positive samples were solely infected with BooV, indicating its significance in NCD in the ROK. All isolates were classified as BooV B in phylogenetic analysis.
This is the first study to determine the prevalence and molecular characteristics of BooV in calf diarrhea in the ROK, highlighting the potential importance of BooV as a causative agent of calf diarrhea and highlighting the need for further research on its epidemiology and pathogenicity.
尽管在畜群管理、饲养和药物干预方面取得了进展，但新生犊牛腹泻（NCD）仍然是一个全球性的主要健康问题。细菌、病毒和寄生虫是 NCD 的主要致病因素。尽管韩国（ROK）已经确定了几种病原体，但许多 NCD 病例的病因仍未确定。
首次确定 BooV 在 ROK 犊牛腹泻中的流行情况和影响。
在这里，使用宏基因组学确定了犊牛腹泻的未知原因。然后，我们探讨了包括 BooV 在内的引起 NCD 的某些病原体的流行情况。使用逆转录酶和定量实时聚合酶链反应对 70 份韩牛犊牛腹泻粪便样本进行病原体检测和 BooV 分离物测序。
从犊牛腹泻的未知原因中检测到 BooV 的完整基因组。并且，BooV 是在 70 份韩牛犊牛腹泻粪便中 8 种病原体中检测到的最常见病原体（35.7%）。共感染分析表明，大多数 BooV 阳性样本仅感染 BooV，表明其在 ROK 中 NCD 的重要性。所有分离株在系统发育分析中均被归类为 BooV B。
这是首次在 ROK 确定 BooV 在犊牛腹泻中的流行情况和分子特征的研究，突出了 BooV 作为犊牛腹泻致病因子的潜在重要性，并强调了进一步研究其流行病学和致病性的必要性。
