同种异体纯富血小板血浆、氯唑西林钠及其联合应用对杂交奶牛亚临床乳腺炎的治疗效果。

Effect of allogeneic pure platelet-rich plasma, sodium cloxacillin, and their combination for the treatment of subclinical mastitis in crossbred cows.

作者信息

López Catalina, Duque-Madrid Paulo Cesar, Ceballos-Márquez Alejandro, Carmona Jorge U

机构信息

Grupo de Investigación Patología Clínica Veterinaria, Departamento de Salud Animal, Universidad de Caldas, Manizales, Colombia.

Grupo de Investigación Calidad de Leche y Epidemiología Veterinaria, Departamento de Producción Agropecuaria, Universidad de Caldas, Manizales, Colombia.

出版信息

Front Vet Sci. 2024 Aug 22;11:1432354. doi: 10.3389/fvets.2024.1432354. eCollection 2024.

INTRODUCTION

Bovine subclinical mastitis (SCM) caused by Gram-positive bacteria is a major cause of economic loss in the dairy industry, exacerbated in situations where antimicrobial resistance is present. Pure platelet-rich plasma (P-PRP) may be a therapeutic alternative for SCM, when used alone or with antibiotics, such as sodium cloxacillin (SC). This study aimed 1) to evaluate the therapeutic efficacy of allogeneic P-PRP, SC, and their combination (P-PRP+SC) in cows with SCM caused by and by streptococci ( and ); 2) to determine the concentrations of somatic cells (SCC), interleukin 1 beta (IL-1β), tumor necrosis factor-alpha (TNF-α) and TGF-β in milk samples of the cows.

METHODS

130 cows from 4 dairy herds completed the study, of which 40 cows were treated with P-PRP (10 mL), 28 cows with SC (5g), 36 with P-PRP+SC (10mL/5g), and 26 did not receive no treatment (negative control group, NCG).

RESULTS

The overall bacteriological cure was observed in 10/40 (25%) cows in the P-PRP group, 9/28 (32.14%) animals in the SC group, 26/36 (72.22%) cows in the P-PRP+SC group, and 10/26 (38.46%) animals in the NCG. SCM caused by (82/130, 63.08%), was cured in 6/24 (25%) cows treated with P-PRP, 7/24 (29.2%) cows treated with SC, 8/16 (50%) animals treated with P-PRP+SC, and in 8/18 (44.4%) cows in NCG. For SCM caused by the streptococci (48/130, 36.91%), the cure was achieved in 4/12 (33.3%) cows treated with P-PRP, 2/4 (50%) cows treated with SC, 18/20 (90%) cows treated with P-PRP+SC, and in 2/8 (25%) cows of the NCG. SCC was significantly ( < 0.001) affected by the treatment, herd, cure, bacteria group, and number of calvings factors. IL-1β milk concentrations were significantly ( < 0.001) influenced by treatment and farm factors, and the interaction between these factors. TNF-α milk concentrations were significantly ( < 0.001) influenced by time factor. TGF-β milk concentrations were significantly affected by the time and cure factors.

CONCLUSION

The combination of P-PRP and SC showed the best therapeutic response (90%) against bovine SCM caused by streptococci. However, none of the treatments showed an effective therapeutic response against .

引言

由革兰氏阳性菌引起的牛亚临床型乳腺炎（SCM）是乳制品行业经济损失的主要原因，在存在抗生素耐药性的情况下，情况会更加恶化。纯富血小板血浆（P-PRP）单独使用或与抗生素（如氯唑西林钠（SC））联合使用时，可能是治疗SCM的一种替代方法。本研究旨在：1）评估同种异体P-PRP、SC及其组合（P-PRP+SC）对由金黄色葡萄球菌和链球菌（无乳链球菌和停乳链球菌）引起的SCM奶牛的治疗效果；2）测定奶牛乳汁样本中体细胞（SCC）、白细胞介素1β（IL-1β）、肿瘤坏死因子-α（TNF-α）和转化生长因子-β（TGF-β）的浓度。

方法

来自4个奶牛场的130头奶牛完成了本研究，其中40头奶牛接受P-PRP（10 mL）治疗，28头奶牛接受SC（5g）治疗，36头奶牛接受P-PRP+SC（10mL/5g）治疗，26头奶牛未接受任何治疗（阴性对照组，NCG）。

结果

P-PRP组10/40（25%）的奶牛实现了总体细菌学治愈，SC组9/28（32.14%）的动物实现了总体细菌学治愈，P-PRP+SC组26/36（72.22%）的奶牛实现了总体细菌学治愈，NCG组10/26（38.46%）的动物实现了总体细菌学治愈。由金黄色葡萄球菌引起的SCM（82/130，63.08%），接受P-PRP治疗的6/24（25%）奶牛、接受SC治疗的7/24（29.2%）奶牛、接受P-PRP+SC治疗的8/16（50%）动物以及NCG组8/18（44.4%）的奶牛得到治愈。对于由链球菌引起的SCM（48/130，36.91%），接受P-PRP治疗的4/12（33.3%）奶牛、接受SC治疗的2/4（50%）奶牛、接受P-PRP+SC治疗的18/20（90%）奶牛以及NCG组2/8（25%）的奶牛得到治愈。SCC受到治疗、牛群、治愈情况、细菌种类和产犊次数等因素的显著影响（P<0.001）。IL-1β乳汁浓度受到治疗和农场因素以及这些因素之间相互作用的显著影响（P<0.001）。TNF-α乳汁浓度受到时间因素的显著影响（P<0.001）。TGF-β乳汁浓度受到时间和治愈情况因素的显著影响。