Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Ontario, Canada.
Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ontario, Canada.
Microbiol Spectr. 2024 Oct 3;12(10):e0150924. doi: 10.1128/spectrum.01509-24. Epub 2024 Sep 6.
(ACB) complex has been identified as a group of emerging opportunistic pathogens that cause nosocomial infections. The current study investigates the prevalence, distribution, and diversity of pathogenic ACB complex in various aquatic systems with different uses. Of the total 157 agricultural, raw drinking water intake, recreational beach, and wastewater treatment plant (WWTP) effluent samples, acinetobacters were isolated, quantified, and confirmed by genus- and ACB complex-specific PCR assays. Of all agricultural surface water samples, (65%) was more frequently detected than (14%), (9%), and (3%). In WWTP effluent samples, was more prevalent in de-chlorinated (60%) samples compared to both and (40%). Interestingly (43%), (29%), and (14%) were detected in raw drinking water intake samples, whereas (50%) and (25%) were detected in beach samples. Although no sampling location-specific differences were recorded, significant ( < 0.05) seasonal differences were observed when agricultural surface water samples collected in spring were compared with the summer and fall. Whereas effluent chlorination significantly impacted the degree of prevalence of in WWTP effluent samples, overall, the prevalence of ACB complex in all sampling locations and seasons indicates that these water sources, containing human-associated ACB complex, may pose potential health risks as community-acquired opportunistic infections.IMPORTANCE (ACB) complex is a group of organisms known to cause problematic nosocomial opportunistic infections. A member of the species complex, , is becoming a global threat to infection treatment as strains are increasingly develop resistance to antibiotics. The prevalence and distribution of potentially pathogenic complex species remain poorly understood, and there is a need to better understand the occurrence of in non-nosocomial environments. Our research details the spatial-temporal distribution of ACB complex species in a regional watershed and highlights the presence of ACB complex in wastewater effluent that is discharged into a river. These findings deepen our understanding of this group of species in non-nosocomial environments and encourage the development of monitoring programs for these species in regional waters.
(ACB) 复合体已被确定为一组新兴的机会性病原体，可引起医院感染。本研究调查了具有不同用途的各种水生系统中致病性 ACB 复合体的流行率、分布和多样性。在总共 157 个农业、原饮用水摄入、娱乐海滩和污水处理厂 (WWTP) 污水样本中，通过属和 ACB 复合体特异性 PCR 检测分离、定量并确认了不动杆菌。在所有农业地表水样本中，(65%)比(14%)、(9%)和(3%)更频繁地被检测到。在 WWTP 污水样本中，在脱氯(60%)样本中比(40%)更普遍。有趣的是，(43%)、(29%)和(14%)在原饮用水摄入样本中被检测到，而(50%)和(25%)在海滩样本中被检测到。尽管没有记录到采样地点特异性差异，但当比较春季采集的农业地表水样本与夏季和秋季时，观察到显著的( < 0.05)季节性差异。虽然污水氯化显著影响 WWTP 污水样本中 的流行程度，但总体而言，所有采样地点和季节的 ACB 复合体的流行率表明，这些水源含有与人相关的 ACB 复合体，可能构成社区获得性机会性感染的潜在健康风险。重要性 (ACB) 复合体是一组已知会引起有问题的医院获得性机会性感染的生物体。该物种复合体的一个成员， ，作为一种对感染治疗构成威胁的全球威胁，因为越来越多的菌株对抗生素产生耐药性。潜在致病性 ACB 复合体物种的流行率和分布仍知之甚少，需要更好地了解非医院环境中 的发生情况。我们的研究详细说明了 ACB 复合体物种在区域流域中的时空分布，并强调了在排入河流的污水中存在 ACB 复合体。这些发现加深了我们对这些非医院环境中物种组的理解，并鼓励在区域水域中开发这些物种的监测计划。
