生成 ESC 衍生的 RGC 用于细胞替代治疗。

Generating ESC-Derived RGCs for Cell Replacement Therapy.

机构信息

Department of Ophthalmology, Louis J. Fox Center for Vision Restoration, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.

Department of Neurobiology, Center of Neuroscience, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.

出版信息

Methods Mol Biol. 2025;2848:187-196. doi: 10.1007/978-1-0716-4087-6_12.

DOI:10.1007/978-1-0716-4087-6_12

PMID:39240524

Abstract

In several ocular diseases, degeneration of retinal neurons can lead to permanent blindness. Transplantation of stem cell (SC)-derived RGCs has been proposed as a potential therapy for RGC loss. Although there are reports of successful cases of SC-derived RGC transplantation, achieving long-distance regeneration and functional connectivity remains a challenge. To address these hurdles, retinal organoids are being used to study the regulatory mechanism of stem cell transplantation. Here we present a modified protocol for differentiating human embryonic stem cells (ESCs) into retinal organoids and transplanting organoid-derived RGCs into the murine eyes.

摘要

在一些眼部疾病中，视网膜神经元的退化可能导致永久性失明。干细胞（SC）衍生的 RGC 移植已被提议作为治疗 RGC 损失的一种潜在疗法。尽管有报道称 SC 衍生的 RGC 移植成功的案例，但实现远距离再生和功能连接仍然是一个挑战。为了解决这些障碍，视网膜类器官正被用于研究干细胞移植的调节机制。在这里，我们提出了一种改良的方案，用于将人胚胎干细胞（ESCs）分化为视网膜类器官，并将类器官衍生的 RGC 移植到小鼠眼中。