Department of Biology, The University of Akron, Akron, Ohio.
CellSight Ocular Stem Cell and Regeneration Program, Sue Anschutz-Rodgers Eye Center, University of Colorado School of Medicine, Aurora, Colorado.
Transl Res. 2022 Dec;250:98-111. doi: 10.1016/j.trsl.2022.06.001. Epub 2022 Jun 9.
The ability to generate human retinas in vitro from pluripotent stem cells opened unprecedented opportunities for basic science and for the development of therapeutic approaches for retinal degenerative diseases. Retinal organoid models not only mimic the histoarchitecture and cellular composition of the native retina, but they can achieve a remarkable level of maturation that allows them to respond to light stimulation. However, studies evaluating the nature, magnitude, and properties of light-evoked responsivity from each cell type, in each retinal organoid layer, have been sparse. In this review we discuss the current understanding of retinal organoid function, the technologies used for functional assessment in human retinal organoids, and the challenges and opportunities that lie ahead.
从多能干细胞体外生成人类视网膜为基础科学和开发治疗视网膜退行性疾病的方法提供了前所未有的机会。视网膜类器官模型不仅模拟了天然视网膜的组织架构和细胞组成，而且可以达到惊人的成熟水平，使其能够对光刺激产生反应。然而，评估每个视网膜类器官层中每种细胞类型的光诱发反应的性质、幅度和特性的研究还很少。在这篇综述中，我们讨论了对视网膜类器官功能的现有理解、用于人类视网膜类器官功能评估的技术，以及未来的挑战和机遇。
