Department of Food Science, University of Massachusetts, Amherst, Massachusetts 01003, United States.
J Agric Food Chem. 2024 Sep 18;72(37):20410-20418. doi: 10.1021/acs.jafc.4c03828. Epub 2024 Sep 6.
Curcumin is widely recognized for its health benefits, though the role of gut microbiota in its metabolic transformation was not well studied. In this study, bacterial strains capable of metabolizing curcumin were isolated from human stool samples. Using 16S rRNA and whole-genome sequencing, two novel strains ( UMA_cur1 and UMA_cur2) were identified. In addition, the metabolic products were analyzed using liquid chromatography-mass spectrometry. These strains efficiently converted curcumin into dihydro-curcumin (DHC) and tetrahydro-curcumin (THC). Notably, UMA_cur2 also produced hexahydro-curcumin (HHC) and octahydro-curcumin (OHC), marking the first identification of a strain capable of such transformations. The absence of the YncB gene (typically involved in curcumin conversion) in UMA_cur1 suggests an alternative metabolic pathway. Curcumin metabolism begins during the stationary growth phase, indicating that it is not crucial for primary growth functions. Furthermore, UMA_cur2 produced these metabolites sequentially, starting with DHC and THC and progressing to HHC and OHC. These findings identified two novel strains that can metabolize curcumin to hydrogenated metabolites, which enhance our understanding of the interaction between curcumin and gut microbiota.
姜黄素因其对健康的益处而被广泛认可，尽管其在代谢转化过程中肠道微生物群的作用尚未得到充分研究。在这项研究中，从人类粪便样本中分离出能够代谢姜黄素的细菌菌株。使用 16S rRNA 和全基因组测序，鉴定出两种新型菌株（UMA_cur1 和 UMA_cur2）。此外，还使用液相色谱-质谱法分析了代谢产物。这些菌株能够有效地将姜黄素转化为二氢姜黄素（DHC）和四氢姜黄素（THC）。值得注意的是，UMA_cur2 还产生了六氢姜黄素（HHC）和八氢姜黄素（OHC），这标志着首次鉴定出能够进行这种转化的菌株。UMA_cur1 中不存在 YncB 基因（通常参与姜黄素转化）表明存在替代代谢途径。姜黄素代谢始于静止生长阶段，表明其对于初始生长功能并非至关重要。此外，UMA_cur2 依次产生这些代谢物，从 DHC 和 THC 开始，然后转化为 HHC 和 OHC。这些发现确定了两种新型菌株，它们可以将姜黄素代谢为氢化代谢物，这增强了我们对姜黄素与肠道微生物群相互作用的理解。
