Department of Food Science, University of Massachusetts, Amherst, MA 01003, USA.
Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou, Guangdong, China.
Food Chem. 2024 Dec 1;460(Pt 2):140706. doi: 10.1016/j.foodchem.2024.140706. Epub 2024 Jul 30.
Curcumin might exert its therapeutic effects by interacting with gut microbiota. However, the role of gut microbiota in curcumin metabolism in vivo remains poorly understood. To address this, we used antibiotics to deplete gut microbiota and compared curcumin metabolism in control and antibiotic-treated mice. Using Q-TOF and triple quadrupole mass spectrometry, we identified and quantified curcumin metabolites, revealing distinct metabolic pathways in these two mice groups. The novel metabolites, hexahydro-dimethyl-curcumin and hexahydro-didemethyl-curcumin were exclusively derived from gut microbiota. Additionally, gut bacteria deconjugated curcumin metabolites back into their bioactive forms. Moreover, control mice exhibited significantly lower curcumin degradation, suggesting a protective role of gut microbiota against degradation. In conclusion, our results indicated that gut microbiota might enhance the effectiveness of curcumin by deconjugation, production of active metabolites, and protection against degradation in the large intestine. This study enhances our understanding of the interactions between curcumin and gut microbiota.
姜黄素可能通过与肠道微生物群相互作用发挥其治疗作用。然而，肠道微生物群在体内姜黄素代谢中的作用仍知之甚少。为了解决这个问题，我们使用抗生素来耗尽肠道微生物群，并比较了对照组和抗生素处理组小鼠的姜黄素代谢。使用 Q-TOF 和三重四极杆质谱，我们鉴定和定量了姜黄素代谢物，揭示了这两组小鼠中不同的代谢途径。新型代谢物六氢二甲基姜黄素和六氢二去甲姜黄素仅源自肠道微生物群。此外，肠道细菌将姜黄素代谢物去共轭回它们的生物活性形式。此外，对照组小鼠的姜黄素降解明显较低，表明肠道微生物群对降解具有保护作用。总之，我们的结果表明，肠道微生物群可能通过去共轭、产生活性代谢物以及在大肠中防止降解来增强姜黄素的有效性。这项研究增进了我们对姜黄素和肠道微生物群相互作用的理解。
