School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, 110016, PR China.
School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, 110016, PR China.
Biosens Bioelectron. 2024 Dec 15;266:116682. doi: 10.1016/j.bios.2024.116682. Epub 2024 Aug 19.
The development of an affordable, portable, and instrument-free colorimetric biosensor holds significant importance for routine monitoring and clinical diagnosis. To overcome the limitations that traditional monochromatic colorimetric kits struggle to distinguish subtle color changes with the naked eye, we designed and constructed a portable hydrogel kit for polychromatic semi-quantitative and quantitative sensing analysis. When the actual samples and I- were introduced into a gelatin hydrogel encapsulated with MIL-88A(Fe), Au NRs and oxidase (Au@GM88A/I), a noticeable color change occurred. Additionally, a mathematic model between Hue and multicolor signal was set up for the first time by mobile phone photo technology, successfully applied to the glucose detection in serum. The visual detection had a wide concentration range of 0.02-0.80 mM with a limit of detection down to 0.02 mM. Above all, hydrogel kit prepared with gelatin as a carrier addressed the issues of uneven color and slow response rate commonly seen in gels like sodium alginate and agarose. This improvement would be beneficial for enhancing the accuracy of color captured by mobile phone assisted hydrogel kits, making it a valuable tool for biomarker analysis.
开发一种价格合理、便携且无需仪器的比色生物传感器对于常规监测和临床诊断具有重要意义。为了克服传统单色比色试剂盒难以用肉眼区分细微颜色变化的局限性，我们设计并构建了一种用于多色半定量和定量传感分析的便携式水凝胶试剂盒。当实际样品和 I-被引入到封装有 MIL-88A(Fe)、Au NRs 和氧化酶 (Au@GM88A/I) 的明胶水凝胶中时，会发生明显的颜色变化。此外，我们首次通过手机拍照技术建立了色调与多色信号之间的数学模型，成功应用于血清中的葡萄糖检测。该可视化检测具有 0.02-0.80mM 的宽浓度范围，检测限低至 0.02mM。最重要的是，以明胶为载体的水凝胶试剂盒解决了海藻酸钠和琼脂糖等凝胶中常见的颜色不均匀和响应速度慢的问题。这种改进将有助于提高手机辅助水凝胶试剂盒捕捉颜色的准确性，使其成为生物标志物分析的有价值工具。
