Karasov Talia L, Neumann Manuela, Leventhal Laura, Symeonidi Efthymia, Shirsekar Gautam, Hawks Aubrey, Monroe Grey, Exposito-Alonso Moisés, Bergelson Joy, Weigel Detlef, Schwab Rebecca
School of Biological Sciences, University of Utah, Salt Lake City, UT, USA.
Department of Molecular Biology, Max Planck Institute for Biology Tübingen, Tübingen, Germany.
Nat Microbiol. 2024 Oct;9(10):2748-2758. doi: 10.1038/s41564-024-01773-z. Epub 2024 Sep 6.
Plants are colonized by distinct pathogenic and commensal microbiomes across different regions of the globe, but the factors driving their geographic variation are largely unknown. Here, using 16S ribosomal DNA and shotgun sequencing, we characterized the associations of the Arabidopsis thaliana leaf microbiome with host genetics and climate variables from 267 populations in the species' native range across Europe. Comparing the distribution of the 575 major bacterial amplicon variants (phylotypes), we discovered that microbiome composition in A. thaliana segregates along a latitudinal gradient. The latitudinal clines in microbiome composition are predicted by metrics of drought, but also by the spatial genetics of the host. To validate the relative effects of drought and host genotype we conducted a common garden field study, finding 10% of the core bacteria to be affected directly by drought and 20% to be affected by host genetic associations with drought. These data provide a valuable resource for the plant microbiome field, with the identified associations suggesting that drought can directly and indirectly shape genetic variation in A. thaliana via the leaf microbiome.
在全球不同地区，植物会被不同的致病微生物群落和共生微生物群落定殖，但驱动其地理变异的因素在很大程度上尚不清楚。在这里，我们利用16S核糖体DNA和鸟枪法测序，对欧洲拟南芥原生范围内267个种群的拟南芥叶微生物群落与宿主遗传学和气候变量之间的关联进行了表征。通过比较575种主要细菌扩增子变体（系统型）的分布，我们发现拟南芥的微生物群落组成沿纬度梯度分离。微生物群落组成的纬度梯度变化可由干旱指标预测，也可由宿主的空间遗传学预测。为了验证干旱和宿主基因型的相对影响，我们进行了一项普通园圃田间研究，发现10%的核心细菌直接受到干旱影响，20%受到宿主与干旱的遗传关联影响。这些数据为植物微生物群落领域提供了宝贵资源，所确定的关联表明干旱可通过叶微生物群落直接和间接地塑造拟南芥的遗传变异。
