College of Food Science and Nutritional Engineering, Beijing Key Laboratory of Viticulture and Enology, China Agricultural University, Beijing, 100083, PR China.
Biochem Biophys Res Commun. 2021 May 21;554:193-198. doi: 10.1016/j.bbrc.2021.03.092. Epub 2021 Mar 31.
WRKY transcription factors are one of the largest transcriptional regulator families, involved in various signaling networks in plants. However, only limited functional exploration of the sugar signaling of Vitis vinifera WRKY22 transcription factor (VvWRKY22) has been conducted. In this study, the roles played by VvWRKY22 in sugar accumulation in grapes were investigated. VvWRKY22 was co-expressed with 16 sugar-related genes, and the expression of VvWRKY22 in grape suspension cells was inhibited by sucrose, and induced by fructose and abscisic acid (ABA). Results showed that over-expression of VvWRKY22 decreased the sucrose, glucose and fructose content, and regulated the expression levels of sugar and ABA-related genes. Moreover, it was found that VvWRKY22 interacted with VvSnRK1.1 or VvSnRK1.2 proteins (Sucrose non-fermenting-1-related protein kinase 1), which are important kinases related to sugar metabolism. These results, thus, provide new genetic evidences to support the view that VvWRKY22 functions in regulating sugar metabolism in grapes.
WRKY 转录因子是最大的转录调控因子家族之一，参与植物中的各种信号网络。然而，对葡萄 Vitis vinifera WRKY22 转录因子（VvWRKY22）的糖信号的功能研究还很有限。在本研究中，研究了 VvWRKY22 在葡萄中糖积累中的作用。VvWRKY22 与 16 个糖相关基因共表达，葡萄悬浮细胞中的 VvWRKY22 表达被蔗糖抑制，被果糖和脱落酸（ABA）诱导。结果表明，过表达 VvWRKY22 降低了蔗糖、葡萄糖和果糖的含量，并调节了糖和 ABA 相关基因的表达水平。此外，还发现 VvWRKY22 与 VvSnRK1.1 或 VvSnRK1.2 蛋白（蔗糖非发酵-1 相关蛋白激酶 1）相互作用，这些蛋白是与糖代谢相关的重要激酶。这些结果为 VvWRKY22 在调节葡萄糖代谢中的功能提供了新的遗传证据。
