Disturbance of testosterone cycle in favism-induced male rats is prevented by pracaxi oil oral administration.

OBJECTIVES

Favism is a metabolic disease while pracaxi oil is a strong antioxidant agent. This study evaluates anti-infertility activity and assists normal male fertilization of pracaxi oil in favism-induced male rats.

METHODS

A total of 36 male albino rats of six equal sets were each with 6 rats; Control, Pracaxi oil (1 mL), and Pracaxi oil (2 mL), Favism-induced male rats-, Pracaxi oil (1 mL) + Favism-induced male rats, and Pracaxi oil (2 mL) + Favism-induced male rats groups. Blood parameters, liver function, serum male hormones were determined. Glucose-6-phosphate dehydrogenase, 3β-hydroxysteroid dehydrogenase, total protein, and cholesterol in testis were estimated. Sodium/potassium-ATPase and antioxidants in the hypothalamus, testis, and sperm were assessed. Sperm count, motility, and abnormality, and sperm monoclonal proliferating antibody Ki-67 were evaluated.

RESULTS

Favism decreased blood parameters, liver function, superoxide dismutase, glutathione, serum testosterone and dehydroepiandrosterone sulfate, sperm count and motility, sodium/potassium-ATPase activity while increased malondialdehyde, serum follicle stimulating hormone, sex hormone binding globulin, and luteinizing hormone, glucose-6-phosphatedehydrogenase, 3β-hydroxysteroid dehydrogenase, cholesterol, total protein, sperm abnormality, the percentage of spermatogonia, 1st spermatocyte, 2nd spermatocyte, and spermatid in the testis. Furthermore, two doses of pracaxi oil to favism-induced male rats back all of aforementioned parameters to be close control values where a higher dose of pracaxi oil had an efficient impact than a lower dose.

CONCLUSIONS

Pracaxi oil protects the hypothalamic-pituitary-gonad axis, and preserves sperm quality in favism-induced male rats.