Koriem Khaled M M, El-Masry Mayar S R
Department of Medical Physiology, Medical Research and Clinical Studies Institute, National Research Centre, El-Buhouth Street, P.O. Box. 12622, Dokki, Giza, Egypt.
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, P.O. Box.11562, Cairo, Egypt.
Toxicol Rep. 2024 Nov 29;13:101845. doi: 10.1016/j.toxrep.2024.101845. eCollection 2024 Dec.
Plastic products use phthalate to enhance their flexibility, transparency, and stability, while behenic acid is a carboxylic acid with antioxidant activity.
This study evaluates whether behenic acid can protect the testosterone cycle and prevent the sperm apoptosis and protein loss in phthalate-treated male rats.
There were 36 male albino rats in all, divided into six equal sets of six rats each: control, behenic acid (13 g/kg), behenic acid (26 g/kg), diethyl phthalate (10 mg/kg), behenic acid (13 g/kg) + diethyl phthalate (10 mg/kg), and behenic acid (26 g/kg) + diethyl phthalate (10 mg/kg)-treated groups. Measurements were made of serum male hormones, sex hormone-binding globulin, sodium/potassium ATPase, superoxide dismutase, glutathione, glucose-6-phosphate dehydrogenase, 3β-hydroxysteroid dehydrogenase, protein, and cholesterol in the testis, as well as malondialdehyde in the sperm, testis, and hypothalamus. Sperm monoclonal proliferating antibody Ki-67, sperm counts, motility, and abnormalities were measured.
Oral administration of diethyl phthalate increased malondialdehyde, serum follicle stimulating hormone, sex hormone binding globulin, luteinizing hormone, glucose-6-phosphate dehydrogenase, 3β-hydroxysteroid dehydrogenase, cholesterol, total protein, sperm abnormality, and the percentage of spermatogonia, first spermatocyte, second spermatocyte, and spermatid in the testis. Superoxide dismutase, glutathione, serum testosterone and dehydroepiandrosterone sulphate, sperm count and motility, and sodium/potassium-ATPase activity were all reduced. Additionally, all of the previously described parameters reverted to near control values after receiving two doses of behenic acid in phthalate-treated rats; a higher dose of behenic acid had a more effective effect than a lower dose.
behenic acid can protect the testosterone cycle and prevent the sperm apoptosis and protein loss in phthalate-treated male rats by inhibiting oxidative stress and stimulating ATPase activity.
塑料制品使用邻苯二甲酸盐来增强其柔韧性、透明度和稳定性，而山嵛酸是一种具有抗氧化活性的羧酸。
本研究评估山嵛酸是否能保护睾酮循环，并防止邻苯二甲酸盐处理的雄性大鼠精子凋亡和蛋白质损失。
总共36只雄性白化大鼠，分为六组，每组六只：对照组、山嵛酸（13 g/kg）组、山嵛酸（26 g/kg）组、邻苯二甲酸二乙酯（10 mg/kg）组、山嵛酸（13 g/kg）+邻苯二甲酸二乙酯（10 mg/kg）组和山嵛酸（26 g/kg）+邻苯二甲酸二乙酯（10 mg/kg）处理组。测量血清雄性激素、性激素结合球蛋白、钠/钾ATP酶、超氧化物歧化酶、谷胱甘肽、葡萄糖-6-磷酸脱氢酶、3β-羟基类固醇脱氢酶、睾丸中的蛋白质和胆固醇，以及精子、睾丸和下丘脑中的丙二醛。测量精子单克隆增殖抗体Ki-67、精子数量、活力和异常情况。
口服邻苯二甲酸二乙酯会增加丙二醛、血清促卵泡激素、性激素结合球蛋白、黄体生成素、葡萄糖-6-磷酸脱氢酶、3β-羟基类固醇脱氢酶、胆固醇、总蛋白、精子异常，以及睾丸中生精细胞、初级精母细胞、次级精母细胞和精子细胞的百分比。超氧化物歧化酶、谷胱甘肽、血清睾酮和硫酸脱氢表雄酮、精子数量和活力以及钠/钾-ATP酶活性均降低。此外，在邻苯二甲酸盐处理的大鼠接受两剂山嵛酸后，所有上述参数均恢复到接近对照值；较高剂量的山嵛酸比较低剂量更有效。
山嵛酸可通过抑制氧化应激和刺激ATP酶活性，保护邻苯二甲酸盐处理的雄性大鼠的睾酮循环，并防止精子凋亡和蛋白质损失。
