Department of Instrumentation and Electronics Engineering, Jadavpur University, Kolkata, 700106, India.
University of Engineering and Management, Kolkata, 700160, India.
Anal Chim Acta. 2024 Oct 9;1325:343120. doi: 10.1016/j.aca.2024.343120. Epub 2024 Aug 17.
The present study elucidates the effectiveness of a molecularly imprinted polyacrylonitrile-imbued graphite-base electrode (MAN@G) for the selective detection of folic acid (FA) in food samples. The prime objective of the recognition and quantification of vitamin compounds like FA is the overall quality assessment of vegetables and fruits. The cost-effective, reproducible, and durable MAN@G electrode has been fabricated using acrylonitrile (AN) as the monomer and FA as the template over graphite-base. The characterization of the synthesized MAN@G electrode material has been accomplished by utilizing UV-visible (UV-vis) spectroscopy and scanning electron microscopy (SEM). A tri-electrode system based on differential pulse voltammetry (DPV) and cyclic voltammetry (CV) techniques was employed to explore the analytical performance of the synthesized electrode. Rigorous analyses divulged that a widespread linearity window could be exhibited by the electrode under an optimized experimental environment, ranging from 20 μM to 400 μM concentrations with an acceptable lower limit of detection (LOD) and limit of quantification (LOQ) of 18 nM, and 60 nM respectively. Additionally, this electrode exhibits high reproducibility, good stability, and high repeatability, with RSD values of 1.72 %, 1.32 %, and 1.19 %, respectively. The detection efficacy of the proposed electrode has been further examined in food extracts, namely orange, spinach, papaya, soybean, and cooked rice, which endorsed high accuracy compared to the high-performance liquid chromatography (HPLC) method. Moreover, the statistical results obtained from the t-test analysis were also satisfactory for the FA concentrations present in those five samples.
本研究阐明了一种基于分子印迹的聚丙烯腈浸渍石墨基电极（MAN@G）在食品样品中选择性检测叶酸（FA）的有效性。识别和定量像 FA 这样的维生素化合物的主要目的是评估蔬菜和水果的整体质量。该电极采用丙烯腈（AN）作为单体，FA 作为模板，在石墨基底上制备，具有成本效益高、重现性好、耐用等特点。利用紫外可见光谱（UV-vis）和扫描电子显微镜（SEM）对合成的 MAN@G 电极材料进行了表征。采用三电极系统，基于差分脉冲伏安法（DPV）和循环伏安法（CV）技术，研究了合成电极的分析性能。严格的分析表明，在优化的实验环境下，电极可以表现出广泛的线性窗口，其浓度范围从 20 μM 到 400 μM，具有可接受的低检测限（LOD）和定量限（LOQ）分别为 18 nM 和 60 nM。此外，该电极还表现出高重现性、良好的稳定性和高重复性，其 RSD 值分别为 1.72%、1.32%和 1.19%。该方法已进一步应用于食品提取物（如橙汁、菠菜、木瓜、大豆和米饭）的检测中，与高效液相色谱（HPLC）法相比，该方法具有较高的准确性。此外，对这五个样品中 FA 浓度进行 t 检验分析的统计结果也是令人满意的。
