Tseng Y C, Burman K D, Baker J R, Wartofsky L
Clin Chem. 1985 Jul;31(7):1131-4.
In this enzyme-linked immunoassay for human thyrotropin (TSH) in unextracted serum we use 96-well immunoenzymometric assay plates, first coated with polyclonal antibody to TSH, then incubated with the serum samples and reacted with mouse monoclonal antibody to human TSH. After incubation with alkaline phosphatase-labeled antibody against mouse IgG, disodium p-nitrophenyl phosphate is added and the color change is measured spectrophotometrically. Assay sensitivity is 0.1 milli-int. unit/L. Cross reactivity with lutropin, follitropin, or choriogonadotropin was negligible. TSH concentrations ranged from 0.4 to 4.1 milli-int. units/L in 43 normal subjects (mean 2.0, SD 1.0), and were uniformly less than 0.3 milli-int. unit/L in 23 patients with hyperthyroidism. Features which make this assay advantageous to the clinical laboratory include ease of set-up, ability to assay many samples at a time, high sensitivity, rapid turnaround time (8 h), and absence of requirements for radioactive materials.
在这种用于检测未提取血清中人类促甲状腺激素（TSH）的酶联免疫测定法中，我们使用96孔免疫酶定量测定板，首先用TSH多克隆抗体包被，然后与血清样本孵育，并与人TSH小鼠单克隆抗体反应。在用碱性磷酸酶标记的抗小鼠IgG抗体孵育后，加入对硝基苯磷酸二钠，并通过分光光度法测量颜色变化。测定灵敏度为0.1毫国际单位/升。与促黄体生成素、促卵泡生成素或绒毛膜促性腺激素的交叉反应可忽略不计。43名正常受试者的TSH浓度范围为0.4至4.1毫国际单位/升（平均2.0，标准差1.0），23名甲状腺功能亢进患者的TSH浓度均低于0.3毫国际单位/升。该测定法对临床实验室有利的特点包括设置简便、能够一次检测多个样本、高灵敏度、快速周转时间（8小时）以及无需放射性材料。
