基于药敏试验算法检测耐碳青霉烯铜绿假单胞菌分离株中碳青霉烯酶的存在。
Determination of the presence of carbapenemase enzymes in carbapenem-resistant Pseudomonas aeruginosa isolates by susceptibility test based algorithm.
机构信息
University of Health Sciences, Hamidiye Etfal Training and Research Hospital, Clinical Microbiology Laboratory, Istanbul, Türkiye.
University of Health Sciences, Hamidiye Etfal Training and Research Hospital, Clinical Microbiology Laboratory, Istanbul, Türkiye.
出版信息
Diagn Microbiol Infect Dis. 2024 Dec;110(4):116495. doi: 10.1016/j.diagmicrobio.2024.116495. Epub 2024 Aug 14.
PURPOSE
Phenotypic methods have been proposed for the detection of carbapenemase production. These tests can have slower turnaround times. With the sensitivity-based algorithm described by Gill et al. will be possible to detect the carbapenemase.
METHODS
Carbapenem-resistant Pseudomonas aeruginosa (CRPA) isolates from three hospitals between January 2017 and December 2021 were included. The modified carbapenemase-inactivation-method(mCIM) and two algorithms were used, defined as "primary algorithm, i.e. ceftazidime and cefepime non-susceptible in addition to imipenem or meropenem resistance" and "secondary algorithm, i.e. ceftolozane/tazobactam non-susceptible in addition to imipenem or meropenem resistance". PCR testing was performed on all isolates.
RESULTS
256 CRPA isolates were included in the study. When the primary or secondary algorithm criteria were applied, there were 173 isolates that met one or both of them. Of these, 29 were CIM-positive isolates.
CONCLUSION
In our study, the use of the algorithm reduced the need for CIM testing by 32 %.
目的
已经提出了表型方法来检测碳青霉烯酶的产生。这些测试可能需要更长的周转时间。通过 Gill 等人描述的基于灵敏度的算法将能够检测碳青霉烯酶。
方法
纳入了 2017 年 1 月至 2021 年 12 月期间来自三家医院的耐碳青霉烯铜绿假单胞菌 (CRPA) 分离株。使用了改良的碳青霉烯酶失活法 (mCIM) 和两种算法，定义为“主要算法，即除了对亚胺培南或美罗培南耐药外，头孢他啶和头孢吡肟也不敏感”和“次要算法，即除了对亚胺培南或美罗培南耐药外，头孢唑烷/他唑巴坦也不敏感”。对所有分离株进行了 PCR 检测。
结果
本研究纳入了 256 株 CRPA 分离株。当应用主要或次要算法标准时，有 173 株符合其中一种或两种标准。其中，29 株为 CIM 阳性分离株。
结论
在我们的研究中，该算法将 CIM 测试的需求减少了 32%。
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