State Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China.
State Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China.
Int J Biol Macromol. 2024 Nov;279(Pt 3):135407. doi: 10.1016/j.ijbiomac.2024.135407. Epub 2024 Sep 6.
Two polysaccharides, PGP-90 and PGP-100 (molecular weights of 7.59 × 10 kDa and 10.48 × 10 kDa, respectively), were isolated from Peach gum using alkaline electrolyte water as an extraction solution. Structural characterization showed that PGP-90 and PGP-100 are AG-II arabinogalactans with β-D-(1 → 6)-Galp as the main chain and 1 → 3 Araf and 1 → 5 Araf branched chains at O-3 and O-4 positions. Animal experiments showed that PGP-90 and PGP-100 significantly improved immune function, enhance the proliferative capacity of lymphocytes and phagocytosis of peritoneal macrophages, and regulated the ratio of lymphocyte subpopulations in S180 tumor-bearing mice. Meanwhile, PGP-90 and PGP-100 promoted the secretion of cytokines (TNF-α, IFN-γ, and IL-2) by activated macrophages and blocked apoptosis at the G1 phase, resulting in tumor suppression rates of 40.80 % and 46.30 % (100 mg/kg), respectively, with PGP-100 demonstrating stronger in vivo anti-tumor activity. The above experimental results indicate that Peach gum polysaccharides have the potential to be functional anti-tumor agents.
两种多糖，PGP-90 和 PGP-100（分子量分别为 7.59×10 kDa 和 10.48×10 kDa），从桃胶中用碱性电解质水作为提取溶液分离得到。结构表征表明，PGP-90 和 PGP-100 是 AG-II 阿拉伯半乳聚糖，其主链为β-D-(1→6)-Galp，在 O-3 和 O-4 位具有 1→3 Araf 和 1→5 Araf 支链。动物实验表明，PGP-90 和 PGP-100 显著提高了免疫功能，增强了淋巴细胞的增殖能力和腹腔巨噬细胞的吞噬作用，并调节了 S180 荷瘤小鼠淋巴细胞亚群的比例。同时，PGP-90 和 PGP-100 促进了活化巨噬细胞细胞因子（TNF-α、IFN-γ 和 IL-2）的分泌，并阻断了 G1 期的细胞凋亡，导致肿瘤抑制率分别为 40.80%和 46.30%（100mg/kg），PGP-100 表现出更强的体内抗肿瘤活性。上述实验结果表明，桃胶多糖具有成为功能性抗肿瘤药物的潜力。
