Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China.
Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, 510640, China.
Carbohydr Polym. 2018 Nov 15;200:408-415. doi: 10.1016/j.carbpol.2018.08.027. Epub 2018 Aug 9.
Arabinogalactans are a source of dietary fiber with health benefits. In this work, two arabinogalactans assigned as AGW and AGS were isolated from Larix principis-rupprechtii, and characterized by gel permeation chromatography (GPC), monosaccharide analysis, methylation analysis and NMR spectroscopy analysis. The average molecular weights of AGW and AGS were 1.53 × 10 and 1.84 × 10 Da, respectively. Methylation analysis and NMR spectra suggested that AGW and AGS have a 1,3-linked Galp backbone, branched at C-6 with 1,6-linked Galp side residues. The Ara residues were substituted at C-6 of 1,6-linked Galp consisting of α-L-Araf-(1→3)-α-L-Araf-(1→6)-β-D-Galp-(1→ and β-L-Arap-(1→6)-β-D-Galp-(1→. Significantly, AGS (0.74%) was shown to contain 25 times more uronic acid than AGW (0.03%), which demonstrated a polyelectrolyte effect. Application of these two polysaccharides to macrophage RAW 264.7 cells was shown to increase nitric oxide (NO) production. These results provide a basis for studying the relationship between the structure and biological activity of arabinogalactans.
阿拉伯半乳聚糖是一种具有健康益处的膳食纤维来源。在这项工作中，从落叶松中分离得到两种阿拉伯半乳聚糖，分别命名为 AGW 和 AGS，并通过凝胶渗透色谱（GPC）、单糖分析、甲基化分析和 NMR 光谱分析进行了表征。AGW 和 AGS 的平均分子量分别为 1.53×10 和 1.84×10 Da。甲基化分析和 NMR 谱表明，AGW 和 AGS 具有 1,3-连接的 Galp 主链，在 C-6 处分支，带有 1,6-连接的 Galp 侧残基。Ara 残基取代了 1,6-连接的 Galp 中的 C-6，由α-L-Araf-(1→3)-α-L-Araf-(1→6)-β-D-Galp-(1→和β-L-Arap-(1→6)-β-D-Galp-(1→组成。值得注意的是，AGS（0.74%）中含有 25 倍于 AGW（0.03%）的酸性基团，表现出聚电解质效应。将这两种多糖应用于巨噬细胞 RAW 264.7 细胞，结果表明它们能增加一氧化氮（NO）的产生。这些结果为研究阿拉伯半乳聚糖的结构与生物活性之间的关系提供了依据。
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