Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen Province, Mitraparp Road, Thailand 40002.
Neglected, Zoonosis and Vector-Borne Disease Research Group, Khon Kaen University, Mitraparp Road, Thailand 40002.
J Parasitol. 2024 Oct 1;110(5):423-427. doi: 10.1645/24-15.
Chlorocresol has antibacterial and antifungal properties, yet its effectiveness in eradicating Acanthamoeba spp. remains unexplored. Acanthamoeba species trophozoites are usually sensitive to biocides, whereas cysts tend to be more resistant. This study aimed to evaluate the cysticidal activity of chlorocresol against Acanthamoeba polyphaga. Chlorocresol concentrations of 0.02, 0.04, and 0.08% were prepared and A. polyphaga cysts were incubated at room temperature (28-37 C) for 1, 24, 48, and 72 hr at each concentration. Cyst viability was evaluated using trypan blue staining and the percentage of nonviable cysts was calculated. For qualification assays, treated cysts were cultured on nonnutrient agar medium coated with Escherichia coli, incubated at 30 C, observed under a stereomicroscope for 30 days, and inoculated into peptone-yeast extract-glucose medium at 30 C for 72 hr. The results revealed that the A. polyphaga cysts were susceptible to 0.02, 0.04, and 0.08% chlorocresol. Chlorocresol made a significant difference in viability (P < 0.001) compared with the nontreated control for the same incubation time. This is the first study to examine the efficacy of chlorocresol against A. polyphaga cysts and it was highly effective. Chlorocresol could thus serve as an alternative chemical disinfectant for the eradication of A. polyphaga cysts as well as a prophylactic against transmission of other pathogenic microorganisms for which Acanthamoeba species can act as a carrier.
氯甲酚具有抗菌和抗真菌特性，但它在根除棘阿米巴属方面的效果尚未得到探索。棘阿米巴属滋养体通常对杀菌剂敏感，而包囊则更具抗性。本研究旨在评估氯甲酚对多棘棘阿米巴的杀囊活性。制备了 0.02%、0.04%和 0.08%的氯甲酚浓度，将 A. polyphaga 包囊在室温（28-37°C）下孵育 1、24、48 和 72 小时，每种浓度孵育 1、24、48 和 72 小时。使用台盼蓝染色评估囊的活力，并计算无活力囊的百分比。对于定性检测，将处理过的包囊接种到涂有大肠杆菌的非营养琼脂培养基上，在 30°C 下孵育，用立体显微镜观察 30 天，并在 30°C 下接种到蛋白胨-酵母提取物-葡萄糖培养基中 72 小时。结果表明，A. polyphaga 包囊对 0.02%、0.04%和 0.08%的氯甲酚敏感。与相同孵育时间的未处理对照组相比，氯甲酚在活力方面有显著差异（P<0.001）。这是首次研究氯甲酚对 A. polyphaga 包囊的疗效，效果非常显著。因此，氯甲酚可以作为一种替代化学消毒剂，用于根除 A. polyphaga 包囊，并预防其他致病性微生物的传播，因为棘阿米巴属可以作为这些微生物的载体。
