Ramphal R, Pyle M
Eur J Clin Microbiol. 1985 Apr;4(2):160-2. doi: 10.1007/BF02013590.
The purpose of this study was to obtain more information on the nature of the macromolecule to which Pseudomonas aeruginosa adheres. Acid-injured tracheal epithelium was treated with trypsin or lipase to determine whether the receptor molecule was a protein or a lipid. Lipase treatment significantly reduced adherence to these cells, whereas trypsin had no effect. Since the receptor appeared to be a lipid containing sialic acid, gangliosides were used to test whether they would inhibit adherence. Crude ganglioside preparations inhibited adherence in a dose-dependent manner when added to the bacteria before exposure to tracheal cells. Lastly, fibronectin, which presumably binds to gangliosides, significantly reduced the adherence of these organisms. According to these findings Pseudomonas aeruginosa appears to adhere to a sialic acid-containing glycolipid on cell surfaces, probably a ganglioside.
本研究的目的是获取更多关于铜绿假单胞菌所黏附的大分子性质的信息。用胰蛋白酶或脂肪酶处理酸损伤的气管上皮，以确定受体分子是蛋白质还是脂质。脂肪酶处理显著降低了对这些细胞的黏附，而胰蛋白酶则没有效果。由于受体似乎是一种含唾液酸的脂质，因此使用神经节苷脂来测试它们是否会抑制黏附。当在细菌接触气管细胞之前加入粗制神经节苷脂制剂时，其以剂量依赖的方式抑制黏附。最后，推测与神经节苷脂结合的纤连蛋白显著降低了这些微生物的黏附。根据这些发现，铜绿假单胞菌似乎黏附于细胞表面含唾液酸的糖脂，可能是神经节苷脂。
