Shin Eunguk, Kim Hee-Tae, Lee Haksoo, Kim Byeongsoo, Park Junhyeong, Park Sujin, Yum Soomin, Kim Seul-Kee, Lee Jae-Myung, Youn BuHyun
Nuclear Science Research Institute, Pusan National University, Busan, Korea.
Department of Naval Architecture and Ocean Engineering, Pusan National University, Busan, Korea.
Anim Cells Syst (Seoul). 2024 Sep 5;28(1):428-438. doi: 10.1080/19768354.2024.2396903. eCollection 2024.
Burn injuries, affecting local skin disruption as well as inducing systemic inflammatory responses, are presented as a global public health problem. To enhance the effects of burn wound healing, treatment must simultaneously regulate both re-epithelialization and hyperinflammation. Extracts of () have shown a potential to enhance skin wound healing through antioxidative properties, immune enhancement, and modulation of inflammatory responses. However, despite its promising application for burn wound healing, specific investigation into -derived compounds for enhancing wound healing has not yet been conducted. In this research, we investigated the burn wound-healing effect of the low-temperature pulverization-specific extract (LPSHE), which could not be detected using the room-temperature grinding method. In a mouse burn model with third-degree burn injuries, LPSHE accelerated re-epithelialization by promoting the increase in F-actin formation and reduced burn-induced ROS levels. Additionally, LPSHE significantly regulated hyperinflammation by reducing pro-inflammatory cytokines. Further investigation into molecular mechanisms using HaCaT keratinocytes also demonstrated beneficial effects on burn wound healing. Taken together, our findings suggested that LPSHE is a promising therapeutic candidate for enhancing burn wound healing. Furthermore, this research underscored the importance of low-temperature pulverization in discovering novel natural compounds from marine organisms.
烧伤不仅会影响局部皮肤破损，还会引发全身炎症反应，是一个全球性的公共卫生问题。为提高烧伤创面愈合效果，治疗必须同时调节再上皮化和过度炎症反应。（）的提取物已显示出通过抗氧化特性、增强免疫和调节炎症反应来促进皮肤伤口愈合的潜力。然而，尽管其在烧伤创面愈合方面有应用前景，但尚未对源自（）的化合物促进伤口愈合进行具体研究。在本研究中，我们研究了低温粉碎特定提取物（LPSHE）的烧伤创面愈合效果，该提取物用室温研磨法无法检测到。在三度烧伤的小鼠烧伤模型中，LPSHE通过促进F-肌动蛋白形成增加来加速再上皮化，并降低烧伤诱导的活性氧水平。此外，LPSHE通过减少促炎细胞因子显著调节过度炎症反应。使用HaCaT角质形成细胞对分子机制的进一步研究也证明了其对烧伤创面愈合的有益作用。综上所述，我们的研究结果表明，LPSHE是一种有前途的促进烧伤创面愈合的治疗候选物。此外，本研究强调了低温粉碎在从海洋生物中发现新型天然化合物方面的重要性。
