Killian C E, Bland C E, Kuzava J M, Nishioka D
Exp Cell Res. 1985 Jun;158(2):519-24. doi: 10.1016/0014-4827(85)90475-6.
Unfertilized Strongylocentrotus purpuratus eggs may be treated with ammonia to initiate maternal DNA replication and the maternal cell cycle. When these eggs are polyspermically fertilized 75 min after the beginning of ammonia treatment, the nuclei of the fertilizing spermatozoa undergo premature chromosome condensation (PCC) in an apparent attempt to conform to the advanced maternal cell cycle. PCC is inhibited if maternal DNA replication is blocked by exposing the eggs to aphidicolin but will proceed if this exposure begins after replication is complete. Additionally, PCC will proceed in ammonia-activated, polyspermically fertilized anucleate merogons in the continuous presence of aphidicolin. These results suggest that the direct inhibitory effect of aphidicolin may well be limited to the replication of DNA and that the unreplicated maternal nucleus itself exerts negative control over the development of chromosome-condensing conditions in the maternal cytoplasm.
未受精的紫海胆卵可用氨处理，以启动母本DNA复制和母本细胞周期。当这些卵在氨处理开始75分钟后多精受精时，受精精子的细胞核会发生早熟染色体凝聚（PCC），显然是为了适应提前的母本细胞周期。如果通过将卵暴露于阿非迪霉素来阻断母本DNA复制，PCC会受到抑制，但如果在复制完成后开始这种暴露，PCC仍会进行。此外，在持续存在阿非迪霉素的情况下，PCC会在氨激活的、多精受精的无核卵块中进行。这些结果表明，阿非迪霉素的直接抑制作用很可能仅限于DNA复制，未复制的母本细胞核本身对母本细胞质中染色体凝聚条件的发展施加负调控。
