Partridge C A, Singh S V, Hong T D, Theodore C, Dao D D, Awasthi Y C
Int J Biochem. 1985;17(3):331-40. doi: 10.1016/0020-711x(85)90208-3.
Six forms of glutathione S-transferases designated as GSH S-transferase I (pI 8.8), II (pI 7.2), III (pI 6.8), IV (pI 6.0), V (pI 5.3) and VI (pI 4.8) have been purified from rat lung. GSH S-transferase I (pI 8.8) is a homodimer of Mr 25,000 subunits; GSH S-transferases II (pI 7.2) and VI (pI 4.8) are homodimers of Mr 22,000 subunits; and GSH S-transferases III (pI 6.8), IV (pI 6.0) and V (pI 5.3) are dimers composed of Mr 23,500 and 22,000 subunits. Immunological properties, peptide fragmentation analysis, and substrate specificity data indicate that Mr 22,000, 23,500 and 25,000, are distinct from each other and correspond to Ya, Yb, and Yc subunits, respectively, of rat liver.
已从大鼠肺中纯化出六种谷胱甘肽S-转移酶，分别命名为谷胱甘肽S-转移酶I（pI 8.8）、II（pI 7.2）、III（pI 6.8）、IV（pI 6.0）、V（pI 5.3）和VI（pI 4.8）。谷胱甘肽S-转移酶I（pI 8.8）是由分子量为25,000的亚基组成的同型二聚体；谷胱甘肽S-转移酶II（pI 7.2）和VI（pI 4.8）是由分子量为22,000的亚基组成的同型二聚体；而谷胱甘肽S-转移酶III（pI 6.8）、IV（pI 6.0）和V（pI 5.3）是由分子量为23,500和22,000的亚基组成的二聚体。免疫特性、肽片段分析和底物特异性数据表明，分子量为22,000、23,500和25,000的亚基彼此不同，分别对应大鼠肝脏的Ya、Yb和Yc亚基。
