Erb Isabel K, Suarez Carolina, Frank Ellinor M, Bengtsson-Palme Johan, Lindberg Elisabet, Paul Catherine J
Applied Microbiology, Department of Chemistry, Lund University, PO Box 124, SE-221 00 Lund, Sweden.
Sweden Water Research AB, Ideon Science Park, Scheelevägen 15, SE-223 70 Lund, Sweden.
FEMS Microbes. 2024 Aug 14;5:xtae024. doi: 10.1093/femsmc/xtae024. eCollection 2024.
Marine sediments have been suggested as a reservoir for pathogenic bacteria, including . The origins, and properties promoting survival of in marine sediments (including osmotolerance, biofilm formation capacity, and antibiotic resistance), have not been well-characterized. Phenotypes and genotypes of 37 isolates from coastal marine sediments were characterized. The isolates were diverse: 30 sequence types were identified that have been previously documented in humans, livestock, and other animals. Virulence genes were found in all isolates, with more virulence genes found in isolates sampled from sediment closer to the effluent discharge point of a wastewater treatment plant. Antibiotic resistance was demonstrated phenotypically for one isolate, which also carried tetracycline resistance genes on a plasmid. Biofilm formation capacity varied for the different isolates, with most biofilm formed by phylogroup B1 isolates. All isolates were halotolerant, growing at 3.5% NaCl. This suggests that the properties of some isolates may facilitate survival in marine environments and can explain in part how marine sediments can be a reservoir for pathogenic . As disturbance of sediment could resuspend bacteria, this should be considered as a potential contributor to compromised bathing water quality at nearby beaches.
海洋沉积物被认为是包括……在内的致病细菌的一个储存库。海洋沉积物中……的起源以及促进其存活的特性（包括渗透压耐受性、生物膜形成能力和抗生素抗性）尚未得到充分表征。对从沿海海洋沉积物中分离出的37株……进行了表型和基因型特征分析。这些分离株具有多样性：鉴定出30种序列类型，这些类型先前已在人类、牲畜和其他动物中记录过。在所有分离株中都发现了毒力基因，在从更靠近污水处理厂废水排放点的沉积物中采样的分离株中发现了更多的毒力基因。一株分离株表现出抗生素抗性，该分离株在质粒上还携带四环素抗性基因。不同分离株的生物膜形成能力各不相同，大多数生物膜由B1菌群分离株形成。所有分离株都具有耐盐性，能在3.5%的氯化钠环境中生长。这表明一些分离株的特性可能有助于在海洋环境中存活，并且可以部分解释海洋沉积物如何成为致病……的储存库。由于沉积物的扰动可能使细菌重新悬浮，这应被视为附近海滩浴场水质受损的一个潜在因素。
