Karpen C W, Cataland S, O'Dorisio T M, Panganamala R V
Diabetes. 1985 Jun;34(6):526-31. doi: 10.2337/diab.34.6.526.
Vitamin E content and biosynthesis of 12-hydroxyeicosatetraenoic acid (12-HETE) have been measured in platelets from type I diabetic subjects and age- and sex-matched, nondiabetic control subjects. Platelets from diabetic subjects synthesized significantly greater quantities of 12-HETE than did platelets from control subjects when 12-HETE synthesis was induced by thrombin or collagen, either in the presence or absence of indomethacin. Platelet conversion of exogenously added arachidonic acid (AA) to 12-HETE was not significantly different between the diabetic and control groups in the absence of indomethacin, although a small but significant increase in the conversion of AA to 12-HETE was present in the diabetic group platelets when indomethacin was added to the reaction. Vitamin E content was significantly reduced in platelets from the diabetic subjects, when compared with platelets from the control subjects, although plasma vitamin E levels were not significantly different between the two groups. Thrombin- and collagen-induced platelet 12-HETE synthesis demonstrated a significant negative linear correlation with platelet vitamin E content when measurements from both diabetic and control groups were combined. The above data suggest a relationship between low vitamin E content and increased 12-HETE synthesis in platelets from type I diabetic subjects.
已对I型糖尿病患者以及年龄和性别匹配的非糖尿病对照者的血小板中维生素E含量和12-羟基二十碳四烯酸（12-HETE）的生物合成进行了测定。当在存在或不存在吲哚美辛的情况下用凝血酶或胶原蛋白诱导12-HETE合成时，糖尿病患者的血小板合成的12-HETE量明显多于对照者的血小板。在不存在吲哚美辛的情况下，糖尿病组和对照组之间外源性添加的花生四烯酸（AA）向12-HETE的血小板转化没有显著差异，尽管当向反应中添加吲哚美辛时，糖尿病组血小板中AA向12-HETE的转化有小幅但显著的增加。与对照者的血小板相比，糖尿病患者的血小板中维生素E含量显著降低，尽管两组之间的血浆维生素E水平没有显著差异。当合并糖尿病组和对照组的测量值时，凝血酶和胶原蛋白诱导的血小板12-HETE合成与血小板维生素E含量呈显著负线性相关。上述数据表明I型糖尿病患者血小板中低维生素E含量与12-HETE合成增加之间存在关联。
