Kumar Amit, Priyadarshini G, Parameswaran Sreejith, Ramesh Ananthakrishnan, Rajappa Medha
Department of Biochemistry, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, IND.
Department of Nephrology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, IND.
Cureus. 2024 Aug 9;16(8):e66494. doi: 10.7759/cureus.66494. eCollection 2024 Aug.
Background Chronic kidney disease (CKD) leads to a progressive decline in renal function, primarily due to deteriorating kidney structures. Vascular calcification is a key effect of CKD. MicroRNAs (miRNAs) play a significant role in the onset and progression of both cardiovascular illness and CKD. Aim The aim of this study was to compare biomarkers of endothelial dysfunction, 25-hydroxyvitamin D (25(OH)D), intact parathyroid hormone (iPTH), miRNA 155, and miRNA 145, in patients with CKD versus controls. Methods We recruited 60 patients with CKD and 60 controls. All participants underwent brachial artery flow-mediated dilatation (FMD). Asymmetric dimethylarginine (ADMA) levels were measured using ELISA. Levels of miRNA 145 and miRNA 155 were quantified using real-time polymerase chain reaction (PCR). Results Serum levels of miRNA 145, miRNA 155, 25(OH)D, and FMD were significantly lower in CKD patients compared to controls. Conversely, serum ADMA and iPTH levels were significantly higher in CKD patients. There was a significant negative association between miRNA 145, miRNA 155, FMD, and 25(OH)D with ADMA and iPTH. Additionally, miRNA 145, miRNA 155, FMD, and 25(OH)D showed a significant positive correlation with estimated glomerular filtration rate (eGFR) and with each other. Conclusion Lower levels of miRNA 145 and miRNA 155 and increased endothelial dysfunction correlate with CKD severity, suggesting an accelerated risk for cardiovascular disease (CVD).
慢性肾脏病（CKD）导致肾功能进行性下降，主要原因是肾脏结构恶化。血管钙化是CKD的一个关键影响。微小RNA（miRNA）在心血管疾病和CKD的发生及发展中起重要作用。目的：本研究旨在比较CKD患者与对照组中内皮功能障碍的生物标志物、25-羟基维生素D（25(OH)D）、全段甲状旁腺激素（iPTH）、miRNA 155和miRNA 145。方法：我们招募了60例CKD患者和60例对照。所有参与者均接受肱动脉血流介导的血管舒张功能（FMD）检测。使用酶联免疫吸附测定法（ELISA）测量不对称二甲基精氨酸（ADMA）水平。使用实时聚合酶链反应（PCR）定量miRNA 145和miRNA 155的水平。结果：与对照组相比，CKD患者血清中miRNA 145、miRNA 155、25(OH)D和FMD水平显著降低。相反，CKD患者血清ADMA和iPTH水平显著升高。miRNA 145、miRNA 155、FMD和25(OH)D与ADMA和iPTH之间存在显著负相关。此外，miRNA 145、miRNA 155、FMD和25(OH)D与估计肾小球滤过率（eGFR）之间以及它们彼此之间均呈显著正相关。结论：miRNA 145和miRNA 155水平降低以及内皮功能障碍增加与CKD严重程度相关，提示心血管疾病（CVD）风险加速。
