Microbiology and Clinical Microbiology Unit, Scienze Clinico, Chirurgiche, Diagnostiche, Pediatriche (SCCDP) Department, University of Pavia, Pavia, Italy.
Clinical Microbiology, Azienda Unità Sanitaria Locale (AUSL) Modena, Modena, Italy.
Front Cell Infect Microbiol. 2024 Aug 6;14:1447933. doi: 10.3389/fcimb.2024.1447933. eCollection 2024.
Fosfomycin (FOS) is an effective antibiotic against multidrug-resistant , but its effectiveness is reducing. Little is known on the current prevalence of FosA enzymes in low-risk pathogens, such as . The aim of the study was the molecular characterization of a carbapenemase- and FosA-producing collected in Italy. AK867, collected in 2023, showed an XDR profile, retaining susceptibility only to colistin. AK867 showed a FOS MIC >128 mg/L by ADM. Based on WGS, AK867 belonged to ST116 and owned a wide resistome, including , KPC-2, and VIM-1. was carried by a conjugative pKPC-CAV1312 plasmid of 320,480 bp, on a novel composite transposon (12,907 bp). FosA3 transposon shared similarities with other -harboring pKPC-CAV1312 plasmids among spp. We report the first case of FosA3 production in clinical carbapenemase-producing ST116. The incidence of FosA3 enzymes is increasing among , affecting even low-virulence pathogens, as .
磷霉素（FOS）是一种对抗多药耐药的有效抗生素，但它的效果正在降低。目前关于低危病原体（如）中 FosA 酶的流行情况知之甚少。本研究的目的是对 2023 年在意大利收集的产碳青霉烯酶和 FosA 的 进行分子特征分析。AK867 表现出 XDR 特征，仅对黏菌素保持敏感性。AK867 在 ADM 下显示 FOS MIC >128mg/L。基于 WGS，AK867 属于 ST116 并拥有广泛的耐药性，包括 KPC-2 和 VIM-1。FosA3 转座子由 320480bp 的可接合 pKPC-CAV1312 质粒携带，位于一个新的复合转座子（12907bp）上。FosA3 转座子与 种属中其他携带 pKPC-CAV1312 质粒的 相似。我们报告了首例临床产碳青霉烯酶 ST116 中 FosA3 产生的情况。FosA3 酶的发生率在 中正在增加，甚至影响到低毒力病原体，如 。
