Isgor Pelin Kubra, Abbasiasl Taher, Das Ritu, Istif Emin, Yener Umut Can, Beker Levent
Department of Biomedical Sciences and Engineering, Koç University Rumelifeneri Yolu Sarıyer Istanbul 34450 Turkey
Department of Mechanical Engineering, Koç University Rumelifeneri Yolu Sarıyer Istanbul 34450 Turkey.
Sens Diagn. 2024 Aug 5;3(10):1743-1748. doi: 10.1039/d4sd00135d. eCollection 2024 Oct 10.
Contact lenses offer a simple, cost-effective, and non-invasive method for real-time analysis of various biomarkers. Electro-chemical sensors are integrated into contact lenses for analysis of various biomarkers. However, they suffer from rigid electronic components and connections, leading to eye irritation and biomarker concentration deviation. Here, a flexible and microfluidic integrated paper-based contact lens for colorimetric analysis of glucose was implemented. Facilitating a three-dimensional (3D) printer for lens fabrication eliminates cumbersome cleanroom processes and provides a simple, batch compatible process. Due to the capillary force of the filter paper, the sample was routed to detection chambers inside microchannels, and it allowed further colorimetric detection. The paper-embedded microfluidic contact lens successfully detects glucose down to 2 mM within ∼10 s. The small dimension of the microfluidic system enables detection of glucose levels as low as 5 μl. The results show the potential of the presented approach to analyze glucose concentration in a rapid manner. It is demonstrated that the fabricated contact lens can successfully detect glucose levels of diabetic patients.
隐形眼镜为各种生物标志物的实时分析提供了一种简单、经济高效且非侵入性的方法。电化学传感器被集成到隐形眼镜中用于分析各种生物标志物。然而，它们存在刚性电子元件和连接问题，会导致眼睛刺激和生物标志物浓度偏差。在此，实现了一种用于葡萄糖比色分析的柔性微流体集成纸质隐形眼镜。使用三维（3D）打印机制造镜片消除了繁琐的洁净室工艺，并提供了一种简单的批量兼容工艺。由于滤纸的毛细作用，样品被输送到微通道内的检测室，并允许进行进一步的比色检测。纸质微流体隐形眼镜在约10秒内成功检测到低至2 mM的葡萄糖。微流体系统的小尺寸能够检测低至5 μl的葡萄糖水平。结果显示了所提出方法快速分析葡萄糖浓度的潜力。已证明所制造的隐形眼镜能够成功检测糖尿病患者的葡萄糖水平。
