Ar Reshaid Amjaad Muhammad, Alshawakir Yasser Abdulathim, Almuayrifi Mohammed A, Al-Attas Omar Salem, BaHammam Ahmed S, Al Khalifah Reem Abdullah
Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
Experimental Surgery and Animal Lab, College of Medicine, King Saud University, Riyadh, 11451, Saudi Arabia.
Nat Sci Sleep. 2024 Sep 2;16:1291-1302. doi: 10.2147/NSS.S465917. eCollection 2024.
We aimed to evaluate the effect of light-dark cycle alteration and soft drink consumption on the acceleration of type 1 diabetes mellitus (T1DM) development among non-obese diabetic (NOD) mice model.
We exposed female NOD and C57BL/6 mice from the age of 5 weeks to either adlib soft drink consumption and/or T20 light-dark cycle alteration until the development of diabetes, or the mice reached the age of 30 weeks. Each group consisted of 7-15 mice. We monitored weight, length, blood glucose level, and insulin autoantibody (IAA) levels weekly.
Out of 75 NOD and 22 C57BL/6 mice, 41 NOD mice developed diabetes, and 6 mice died between 7 and 8 weeks of age. The mean time to development of T1DM among NOD control mice was 20 weeks. The time to development of T1DM was accelerated by two weeks in the NOD mice exposed to light-dark cycle alteration, hazard ratio of 2.65,95th CI (0.70, 10.04) p = 0.15). The other groups developed T1DM, similar to the control group.
There was a trend toward earlier development of T1DM among NOD mice exposed to light-dark cycle alteration, but this difference was not statistically significant. Further studies are needed to confirm our findings using larger sample sizes and different animal species.
我们旨在评估明暗周期改变和软饮料摄入对非肥胖糖尿病（NOD）小鼠模型中1型糖尿病（T1DM）发展加速的影响。
我们将5周龄的雌性NOD和C57BL/6小鼠暴露于随意饮用软饮料和/或T20明暗周期改变的环境中，直至糖尿病发展或小鼠达到30周龄。每组由7 - 15只小鼠组成。我们每周监测体重、体长、血糖水平和胰岛素自身抗体（IAA）水平。
在75只NOD小鼠和22只C57BL/6小鼠中，41只NOD小鼠患糖尿病，6只小鼠在7至8周龄之间死亡。NOD对照小鼠中T1DM发展的平均时间为20周。在暴露于明暗周期改变的NOD小鼠中，T1DM发展时间提前了两周，风险比为2.65，95%置信区间（0.70，10.04），p = 0.15）。其他组发展为T1DM的情况与对照组相似。
暴露于明暗周期改变的NOD小鼠中存在T1DM更早发展的趋势，但这种差异无统计学意义。需要进一步研究以使用更大样本量和不同动物物种来证实我们的发现。
