Institute of Chemistry, Federal University of Uberlândia, 38400-902, Uberlândia, Minas Gerais, Brazil.
Anal Methods. 2024 Oct 17;16(40):6793-6801. doi: 10.1039/d4ay01237b.
Laser scribing is a promising technology for the rapid and large-scale production of low-cost electrochemical sensors from diverse substrates. Polyimide has been the most popular so far because of its low cost, flexibility and capability of generating high-quality porous graphene films, known as laser-induced graphene (LIG). Herein we report the electrochemistry of chloramphenicol (CAP) on LIG electrodes and its determination in honey samples. LIG electrodes were fabricated by the photothermal conversion of sp carbon within the polymeric matrix into sp carbon using a CO laser cutter. The LIG electrode associated with differential pulse voltammetry (DPV) showed good linearity ( > 0.99) in the range from 10 to 160 μmol L with a limit of detection of 1.0 μmol L and good precision (RSD < 5%) for the electrochemical reduction of CAP species. Detection was possible free from the interference of other antibiotics, such as amoxicillin, tetracycline, sulfanilamide, and sulfamethoxazole. Spiked honey samples were analyzed by the standard-addition method and recovery values between 86 and 109% were obtained, which confirmed the absence of sample matrix effects. Therefore, the proposed sensor is an alternative, feasible, low-cost, and powerful analytical tool for the determination of CAP in honey.
激光划线是一种很有前途的技术，可以从各种基质上快速大规模生产低成本电化学传感器。到目前为止，聚酰亚胺因其低成本、灵活性和生成高质量多孔石墨烯薄膜（称为激光诱导石墨烯，LIG）的能力而成为最受欢迎的材料。在此，我们报告了 LIG 电极上氯霉素（CAP）的电化学及其在蜂蜜样品中的测定。LIG 电极是通过 CO 激光切割机将聚合物基质内的 sp 碳光热转化为 sp 碳而制备的。与差分脉冲伏安法（DPV）相关的 LIG 电极在 10 至 160 μmol L 的范围内表现出良好的线性（> 0.99），检测限为 1.0 μmol L，对 CAP 物种电化学还原的精密度良好（RSD < 5%）。检测可以不受其他抗生素（如阿莫西林、四环素、磺胺和磺胺甲恶唑）的干扰。通过标准加入法对加标蜂蜜样品进行分析，得到 86%至 109%的回收率，这证实了样品基质无影响。因此，该传感器是一种替代的、可行的、低成本和强大的分析工具，可用于测定蜂蜜中的 CAP。
