Sahin Zafer, Aktas Osman, Kalkan Omer Faruk, Cuce Gokhan, Alver Ahmet, Sahin Elif, Erdem Seniz, Saglam Neslihan, Solak Gormus Zulfikare Isik, Kutlu Selim
Department of Physiology, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey.
Department of Histology and Embryology, Meram Faculty of Medicine, Necmettin Erbakan University, Konya, Turkey.
Biol Futur. 2024 Dec;75(4):477-486. doi: 10.1007/s42977-024-00241-3. Epub 2024 Sep 9.
Kisspeptin is an endogenous peptide hormone that is the most potent stimulator of the hypothalamo-pituitary-gonadal (HPG) axis. The HPG axis can be suppressed by the activation of the hypothalamo-pituitary-adrenal (HPA) axis. The physiological role of kisspeptin in the interaction of the HPG axis and the HPA axis is not fully understood yet. The purpose of the current study was to investigate the possible effects of peripheral injection (intraperitoneally) of kisspeptin on HPG axis and HPA axis activity as well. Adult male Wistar rats were randomly divided into seven groups as sham (control), kisspeptin (10 nmol), p234 (10 nmol), kisspeptin + p234, kisspeptin + antalarmin (10 mg/kg), kisspeptin + astressin2b (100 μg/kg), and kisspeptin + atosiban (0.250 mg/kg) (n = 10 each group). At the end of the experiment, the hypothalamus, pituitary gland, and serum samples of the rats were collected. Serum follicle-stimulating hormone and luteinizing hormone levels of the kisspeptin, kisspeptin + antalarmin and kisspeptin + astressin2b groups were significantly higher than the control group. Serum testosterone levels were significantly higher in the kisspeptin, kisspeptin + antalarmin, kisspeptin + astressin2b, and kisspeptin + atosiban groups that compared to the control group. There was no a significant difference in corticotropic releasing hormone immunoreactivity in the paraventricular nucleus of the hypothalamus, serum adrenocorticotropic hormone and corticosterone concentrations among all groups. Moreover, no significant difference was found in the concentration of pituitary oxytocin. Our results suggest that peripheral kisspeptin injection induces an activation in the HPG axis, but not in the HPA axis in male rats.
亲吻素是一种内源性肽类激素，是下丘脑 - 垂体 - 性腺（HPG）轴最有效的刺激物。下丘脑 - 垂体 - 肾上腺（HPA）轴的激活可抑制HPG轴。亲吻素在HPG轴与HPA轴相互作用中的生理作用尚未完全明确。本研究的目的是探讨腹腔注射亲吻素对HPG轴和HPA轴活性的可能影响。成年雄性Wistar大鼠随机分为七组：假手术组（对照组）、亲吻素组（10 nmol）、p234组（10 nmol）、亲吻素 + p234组、亲吻素 + 安他乐明组（10 mg/kg）、亲吻素 + 阿片生长抑素2b组（100 μg/kg）、亲吻素 + 阿托西班组（0.250 mg/kg）（每组n = 10）。实验结束时，收集大鼠的下丘脑、垂体和血清样本。亲吻素组、亲吻素 + 安他乐明组和亲吻素 + 阿片生长抑素2b组的血清促卵泡生成素和黄体生成素水平显著高于对照组。与对照组相比，亲吻素组、亲吻素 + 安他乐明组、亲吻素 + 阿片生长抑素2b组和亲吻素 + 阿托西班组的血清睾酮水平显著升高。所有组间下丘脑室旁核促肾上腺皮质激素释放激素免疫反应性、血清促肾上腺皮质激素和皮质酮浓度均无显著差异。此外，垂体催产素浓度也无显著差异。我们的结果表明，外周注射亲吻素可诱导雄性大鼠HPG轴激活，但不影响HPA轴。
