Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control, Guangdong-Hong Kong-Macao Joint Laboratory for Contaminants Exposure and Health, Institute of Environmental Health and Pollution Control, Guangdong University of Technology, Guangzhou 510006, China.
Guangzhou Key Laboratory of Environmental Catalysis and Pollution Control, Guangdong Technology Research Center for Photocatalytic Technology Integration and Equipment Engineering, School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou 510006, China.
Environ Sci Technol. 2024 Oct 8;58(40):17937-17947. doi: 10.1021/acs.est.4c06752. Epub 2024 Sep 9.
Bacterial antibiotic resistance has recently attracted increasing amounts of attention. Here, an artificially antibiotic-resistant bacterial community (ARBC) combined with five different constructed antibiotic-resistant bacteria (ARB) with single antibiotic resistance, namely, kanamycin (KAN), tetracycline (TET), cefotaxime (CTX), polymyxin B (PB), or gentamicin (GEM), was studied for the stress response to photocatalysis. With photocatalytic inactivation, the transfer and diffusion of antibiotic resistance genes (ARGs) in the ARBC decreased, and fewer multidrug-resistant bacteria (MDRB) emerged in aquatic environments. After several days of photocatalytic inactivation or Luria broth cultivation, >90% ARB were transformed to antibiotic-susceptible bacteria by discarding ARGs. Bacteria with double antibiotic resistance were the dominant species (99%) of residual ARB. The changes in ARG abundance varied, decreasing for the GEM and TET resistance genes and increasing for the KAN resistance genes. The change in the antibiotic resistance level was consistent with the change in ARG abundance. Correspondingly, point mutations occurred for the KAN, CTX and PB resistance genes after photocatalytic inactivation, which might be the reason why these genes persisted longer in the studied ARBC. In summary, photocatalytic inactivation could reduce the abundance of some ARGs and inhibit the emergence of MDRB as well as block ARG transfer in the bacterial community in aquatic environments. This work highlights the advantages of long-term photocatalytic inactivation for controlling antibiotic resistance and facilitates a better understanding of bacterial communities in real aquatic environments.
细菌抗生素耐药性最近引起了越来越多的关注。在这里，我们研究了一个人工抗生素耐药细菌群落（ARBC）与五种不同的单一抗生素耐药的构建抗生素耐药细菌（ARB）相结合，分别为卡那霉素（KAN）、四环素（TET）、头孢噻肟（CTX）、多粘菌素 B（PB）或庆大霉素（GEM），以研究其对抗生素光催化的应激反应。随着光催化失活，ARBC 中抗生素耐药基因（ARGs）的转移和扩散减少，水生环境中出现的多药耐药细菌（MDRB）减少。经过几天的光催化失活或 Luria 肉汤培养，通过丢弃 ARGs，>90%的 ARB 被转化为抗生素敏感菌。具有双重抗生素耐药性的细菌是剩余 ARB 的主要（99%）物种。ARGs 丰度的变化不同，GEM 和 TET 耐药基因减少，而 KAN 耐药基因增加。抗生素耐药水平的变化与 ARGs 丰度的变化一致。相应地，光催化失活后 KAN、CTX 和 PB 耐药基因发生点突变，这可能是这些基因在研究的 ARBC 中持续时间更长的原因。综上所述，光催化失活可以降低某些 ARGs 的丰度，抑制 MDRB 的出现，并阻止水生环境中细菌群落中 ARG 的转移。这项工作突出了长期光催化失活控制抗生素耐药性的优势，并有助于更好地理解真实水生环境中的细菌群落。
