Brooks S E, Schneider D L
J Protozool. 1985 May;32(2):330-3. doi: 10.1111/j.1550-7408.1985.tb03060.x.
When amebae were incubated with latex beads, cyanide-insensitive oxygen consumption increased nearly two-fold. This cyanide-insensitive respiration was inhibited by salicylhydroxamate. Furthermore, cell fractionation studies revealed a localization for a portion of the NAD(P)H oxidase activity in phagolysosomes. The presence of low concentrations of divalent metal during fractionation resulted in an increased yield of oxidative activity in the phagolysosome fraction. In addition, the phagolysosome membrane was enriched about two-fold in a b-type cytochrome. These results show that oxidative metabolism in amebae has some striking similarities to the respiratory burst oxidase of neutrophils.
当变形虫与乳胶珠一起孵育时，对氰化物不敏感的耗氧量增加了近两倍。这种对氰化物不敏感的呼吸作用被水杨羟肟酸抑制。此外，细胞分级分离研究揭示了一部分NAD(P)H氧化酶活性定位于吞噬溶酶体中。分级分离过程中低浓度二价金属的存在导致吞噬溶酶体部分氧化活性的产量增加。此外，吞噬溶酶体膜中b型细胞色素的含量约增加了两倍。这些结果表明，变形虫中的氧化代谢与中性粒细胞的呼吸爆发氧化酶有一些显著的相似之处。
