Penniall R, Spitznagel J K
Proc Natl Acad Sci U S A. 1975 Dec;72(12):5012-5. doi: 10.1073/pnas.72.12.5012.
Polymorphonuclear leukocytes isolated from chicken peritoneal exudates have been found to catalyze cyanide-insensitive stimulation of respiration and the hexose monophosphate shunt upon exposure to heat-inactivated Staphylococcus aureus. However, there was no demonstrable formate oxidation concomitant with phagocytosis in either the presence or absence of exogenous catalase. Moreover, chicken polymorphonuclear leukocytes failed to oxidize scopoletin concomitant with phagocytosis in the presence of horseradish peroxidase. While oxygen uptake was increased 2- to 3-fold by the stimulus of phagocytosis, the oxidation of [1-(14)C]glucose was increased approximately 20-fold. The cells contain two mechanisms, a glutathione reductase-glutathione peroxidase system and an NADPH-NAD+ transhydrogenase, each of which is present in sufficient capacity to accommodate the enhanced shunt activity. Although chicken polymorphonuclear leukocytes were found to possess a substantial capacity to catalyze the cyanide-insensitive oxidation of either NADH or NADPH, the total or specific activities of such processes were not demonstrably affected by phagocytosis.
从鸡腹膜渗出物中分离出的多形核白细胞，在暴露于热灭活的金黄色葡萄球菌时，已被发现能催化对氰化物不敏感的呼吸刺激和磷酸己糖途径。然而，无论有无外源性过氧化氢酶，吞噬作用均未伴随可证实的甲酸氧化。此外，在辣根过氧化物酶存在的情况下，鸡多形核白细胞在吞噬作用时未能氧化七叶亭。虽然吞噬作用的刺激使氧摄取增加了2至3倍，但[1-(14)C]葡萄糖的氧化增加了约20倍。这些细胞含有两种机制，一种是谷胱甘肽还原酶-谷胱甘肽过氧化物酶系统，另一种是NADPH-NAD+转氢酶，每种机制都有足够的能力来适应增强的磷酸己糖途径活性。尽管发现鸡多形核白细胞具有催化NADH或NADPH对氰化物不敏感氧化的相当能力，但这些过程的总活性或比活性并未明显受到吞噬作用的影响。
