Burchanowski B J, Knigge K M, Sternberger L A
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6671-4. doi: 10.1073/pnas.76.12.6671.
Immunospecific staining in 100-micron-thick Vibratome section by the unlabeled antibody method resembles Golgi staining and reveals an abundance of luliberin- (luteinizing hormone releasing hormone, LHRH) positive cells and fibers in close contact with the surface of the third ventricle. The polarity of LHRH cells can be seen and processes can be traced for several millimeters. In the medial preoptic and suprachiasmatic areas bipolar LHRH neurons send short stout processes to the ventricular surface and long processes toward the organum vasculosum laminae terminalis. These cells resemble the receptor cells contacting the cerebrospinal fluid that have been described by Vigh and Vigh-Teichmann [Vigh, B. & Vigh-Teichmann, I. (1973) Int. Rev. Cytol. 35, 189-251]. In the septal region some bipolar neurons send both of their processes towards the ventricular surface. LHRH neurons in the nucleus of the anterior commissure and the bed nucleus of the stria terminalis project over the anterior commissure to form a dense plexus of fibers in the subfornical organ. The proximity of LHRH perikarya and fibers to the ventricular surface supports the hypothesis [Knigge, K.M., Joseph, S.A., Scott, D.E. & Jacobs, J.J. (1971) in The Neuroendocrinology of Human Reproduction, eds. Mack, H.C. & Sherman, A.J., (Thomas, Springfield, IL), pp. 6-22.] that the cerebrospinal fluid functions in neuroendocrine control mechanisms.
通过未标记抗体法对100微米厚的振动切片机切片进行免疫特异性染色，类似于高尔基染色，显示出大量与第三脑室表面紧密接触的促黄体素释放激素（LHRH）阳性细胞和纤维。可以看到LHRH细胞的极性，其突起可追踪数毫米。在内侧视前区和视交叉上区，双极LHRH神经元向脑室表面发出短而粗的突起，向终板血管器发出长突起。这些细胞类似于Vigh和Vigh-Teichmann [Vigh, B. & Vigh-Teichmann, I. (1973) Int. Rev. Cytol. 35, 189 - 251]所描述的与脑脊液接触的受体细胞。在隔区，一些双极神经元将其两个突起都伸向脑室表面。前连合核和终纹床核中的LHRH神经元向前连合投射，在穹窿下器官形成密集的纤维丛。LHRH核周体和纤维靠近脑室表面支持了这样一种假说[Knigge, K.M., Joseph, S.A., Scott, D.E. & Jacobs, J.J. (1971) in The Neuroendocrinology of Human Reproduction, eds. Mack, H.C. & Sherman, A.J., (Thomas, Springfield, IL), pp. 6 - 22.]，即脑脊液在神经内分泌控制机制中起作用。
