Baker B L, Yu Y
Anat Rec. 1976 Nov;186(3):343-55. doi: 10.1002/ar.1091860302.
The objective was to determine the distribution of growth hormone-release-inhibiting hormone (somatostatin) in the rat brain using the peroxidase-antiperoxidase immunocytochemical method with antisera prepared against unconjugated, synthetic somatostatin. Somatostatin occurred in low quantity in the organum vasculosum of the lamina terminalis. It was present throughout the full length of the median eminence and occupied the entire width between the tuberoinfundibular sulci. Most somatostatin was located in the dorsal portion of the external lamina, and the amount varied according to the mediolateral position. The bodies labeled for somatostatin were most often granules; occasionally they appeared as clusters of granules that seemed to be membrane-enclosed. Some of these bodies appeared to be portions of axons. Many of the larger bodies were arranged alongside tanycytes, but no label was distributed generally in tanycyte cytoplasm. Somatostatin was highly concentrated in the proximal one-quarter of the infundibular stem and appeared in lower concentration throughout the distal portion of the stem. It was absent from the pars nervosa and pars intermedia of the pituitary gland. The distribution of somatostatin in the median eminence differed considerably from that of gonadotropin-releasing hormone. Somatostatin was identified in the ventromedial and/or dorsomedial hypothalamic nuclei of only two animals. Here it was probably located in axons that terminated on neuronal cell bodies but also may have been present in a restricted portion of the perikaryonal cytoplasm.
目的是采用过氧化物酶-抗过氧化物酶免疫细胞化学方法，使用针对未结合的合成生长抑素制备的抗血清，来确定生长激素释放抑制激素（生长抑素）在大鼠脑中的分布。生长抑素在终板血管器中含量较低。它存在于正中隆起的全长，占据漏斗结节沟之间的整个宽度。大多数生长抑素位于外层的背侧部分，其含量根据内外侧位置而有所不同。标记有生长抑素的物体最常见的是颗粒；偶尔它们表现为似乎被膜包围的颗粒簇。其中一些物体似乎是轴突的一部分。许多较大的物体沿着伸长细胞排列，但标记物一般不在伸长细胞的细胞质中分布。生长抑素高度集中在漏斗柄近端的四分之一处，在漏斗柄的远端部分浓度较低。垂体神经部和中间部没有生长抑素。生长抑素在正中隆起的分布与促性腺激素释放激素的分布有很大差异。仅在两只动物的腹内侧和/或背内侧下丘脑核中发现了生长抑素。在这里，它可能位于终止于神经元细胞体的轴突中，但也可能存在于核周细胞质的有限部分。
