Multi-class analysis of 57 drugs quantitatively in blood and qualitatively in urine by LC-MS-MS to complement comprehensive DFC, DUID, and postmortem testing.

A streamlined LC-MS-MS method utilizing protein precipitation and filtration extraction was developed to consolidate analyses for drug-facilitated crimes (DFCs), postmortem (PM) investigations, and driving under the influence of drug (DUID) testing. Fifty-seven target drug and metabolite analytes eluted in <6 minutes and were compromised of gamma-hydroxybutyric acid precursors (1), hallucinogens (3), muscle relaxants (3), anticonvulsants (7), antidepressants (20), antihistamines (5), antipsychotics (11), antihypertensives and alpha-adrenergics (3), analgesics and anesthetics (3), and miscellaneous (1) in blood (quantitatively) and urine (qualitatively). Limits of detection were set to meet the more challenging sensitivity requirements for DFC and are therefore also suitable for PM investigations and other forensic casework, including DUID. Comprehensive Academy Standards Board / American National Standards Institute (ASB/ANSI) validation was performed, and applicability studies examined 72 proficiency test blood and urine samples, along with 9206 unique blood and urine samples from 5192 authentic forensic cases that resulted in 11,961 positive analytes in samples. By expanding the analytical reach across multiple drug classes through a unified approach and screening a wider number of drugs, the technique can identify substances that might have previously evaded detection, thereby enhancing laboratory efficiency by minimizing the need for multiple tests. When combined with a recently developed in-house method, this integrated testing strategy meets the testing requirements outlined in ASB/ANSI standards and recommendations for DFC, PM, and Tier 1 DUID analyses.